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Sample GSM908154 Query DataSets for GSM908154
Status Public on Mar 04, 2013
Title E176_27_Leptin_2_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_27_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_27
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 4.268025555
10001_at 4.120279829
10002_at 3.505034095
10003_at 2.796822488
100048912_at 3.195188884
10004_at 3.63335552
10005_at 5.04805531
10006_at 5.768606373
10007_at 4.407741723
10009_at 2.367588225
1000_at 2.895288725
10010_at 5.039286174
100126791_at 4.726034293
100128124_at 2.929882681
100128640_at 3.461948766
100129128_at 2.407202816
100129250_at 6.546313371
100129271_at 3.656594776
100129482_at 5.288021804
100129973_at 2.582948535

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908154_Holly_41.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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