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Sample GSM908156 Query DataSets for GSM908156
Status Public on Mar 04, 2013
Title E176_09_Leptin_4_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_09_Leptin_4
treatment: Leptin
weeks of treatment: 4
subject: E176_09
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 3.063766597
10001_at 5.436755656
10002_at 2.620504648
10003_at 2.736016223
100048912_at 2.643259607
10004_at 3.639091582
10005_at 5.363877964
10006_at 7.179325034
10007_at 5.33134663
10009_at 2.34315565
1000_at 2.945014396
10010_at 6.021156607
100126791_at 3.442160454
100128124_at 2.76774864
100128640_at 3.001204539
100129128_at 2.106661664
100129250_at 6.111975293
100129271_at 3.032802243
100129482_at 5.105954253
100129973_at 2.436875578

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908156_Holly_45.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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