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Sample GSM908165 Query DataSets for GSM908165
Status Public on Mar 04, 2013
Title E176_32_Leptin_1_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_32_Leptin_1
treatment: Leptin
weeks of treatment: 1
subject: E176_32
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.64951601
10001_at 6.236025146
10002_at 2.376908918
10003_at 2.443249032
100048912_at 2.544996293
10004_at 3.800559739
10005_at 4.905686433
10006_at 7.518693552
10007_at 5.692024669
10009_at 2.385355367
1000_at 2.64103549
10010_at 5.947041191
100126791_at 2.873771805
100128124_at 2.686109774
100128640_at 3.403041195
100129128_at 2.084423729
100129250_at 5.828320354
100129271_at 2.966182379
100129482_at 5.201879714
100129973_at 2.572819952

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908165_Holly_54.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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