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Sample GSM908167 Query DataSets for GSM908167
Status Public on Mar 04, 2013
Title E176_29_Placebo_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_29_Placebo_3
treatment: Placebo
weeks of treatment: 3
subject: E176_29
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.730810709
10001_at 6.067608045
10002_at 2.301416097
10003_at 2.334483749
100048912_at 2.272494643
10004_at 3.854735368
10005_at 4.655116368
10006_at 7.536816113
10007_at 5.539202942
10009_at 2.217965769
1000_at 2.592637671
10010_at 6.083472007
100126791_at 2.94569178
100128124_at 2.793754339
100128640_at 3.069986687
100129128_at 2.196879693
100129250_at 6.145071756
100129271_at 2.801699147
100129482_at 4.597212883
100129973_at 2.56005557

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908167_Holly_56.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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