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Sample GSM908170 Query DataSets for GSM908170
Status Public on Mar 04, 2013
Title E176_30_Placebo_4_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_30_Placebo_4
treatment: Placebo
weeks of treatment: 4
subject: E176_30
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.635158249
10001_at 5.383889991
10002_at 2.534602182
10003_at 2.492942372
100048912_at 2.728416985
10004_at 3.44153017
10005_at 4.942069213
10006_at 7.316742992
10007_at 5.856519921
10009_at 2.280940319
1000_at 2.761229168
10010_at 6.180439113
100126791_at 3.866860191
100128124_at 2.843068749
100128640_at 3.10710326
100129128_at 2.144090989
100129250_at 5.750295848
100129271_at 2.981451756
100129482_at 4.695211641
100129973_at 2.258957468

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908170_Holly_60.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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