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Sample GSM908172 Query DataSets for GSM908172
Status Public on Mar 04, 2013
Title E176_31_Leptin_1_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_31_Leptin_1
treatment: Leptin
weeks of treatment: 1
subject: E176_31
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 2.7810206
10001_at 4.93756622
10002_at 2.392160341
10003_at 2.434092997
100048912_at 2.806481398
10004_at 5.118775046
10005_at 6.107415938
10006_at 6.593396718
10007_at 6.313275998
10009_at 2.407143527
1000_at 2.806484515
10010_at 5.212010956
100126791_at 3.442160454
100128124_at 3.045837397
100128640_at 3.029240284
100129128_at 2.157684066
100129250_at 6.981331997
100129271_at 3.003116959
100129482_at 3.897001598
100129973_at 2.622437729

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908172_Holly_62.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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