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Sample GSM908174 Query DataSets for GSM908174
Status Public on Mar 04, 2013
Title E176_01_Leptin_4_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_01_Leptin_4
treatment: Leptin
weeks of treatment: 4
subject: E176_01
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.399129848
10000_at 2.588910292
100049716_at 3.754922873
10004_at 4.460690754
10008_at 3.186181017
100093630_at 8.857879862
10009_at 7.691170348
100101467_at 3.307937185
100113407_at 4.753278024
10011_at 5.942253456
100124700_at 1.513477428
100125288_at 3.14163053
100126784_at 1.790305191
100126791_at 1.779273536
100126793_at 2.491266521
100127891_at 2.840065203
100127983_at 2.513772512
100128071_at 3.347682508
100128098_at 1.818435889
100128139_at 1.606947382

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908174_Holly_02.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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