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Sample GSM908175 Query DataSets for GSM908175
Status Public on Mar 04, 2013
Title E176_04_Leptin_2_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_04_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_04
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.561117374
10000_at 2.275304467
100049716_at 3.314659444
10004_at 3.878110968
10008_at 4.07205387
100093630_at 8.33081113
10009_at 7.686708095
100101467_at 3.580991138
100113407_at 5.772270702
10011_at 6.135390145
100124700_at 1.534124921
100125288_at 3.203271645
100126784_at 1.892146755
100126791_at 1.997497798
100126793_at 2.624922137
100127891_at 2.415920788
100127983_at 2.554169357
100128071_at 3.499673096
100128098_at 2.326208169
100128139_at 1.768635532

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908175_Holly_03.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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