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Sample GSM908179 Query DataSets for GSM908179
Status Public on Mar 04, 2013
Title E176_05_Placebo_2_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_05_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_05
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 3.224166594
10000_at 2.280438623
100049716_at 3.586950249
10004_at 2.933079937
10008_at 3.336041403
100093630_at 7.967174317
10009_at 7.361616565
100101467_at 3.057175485
100113407_at 4.446302067
10011_at 6.408294864
100124700_at 2.023822721
100125288_at 2.935214227
100126784_at 1.990705253
100126791_at 3.133407609
100126793_at 2.636090931
100127891_at 2.3015205
100127983_at 2.920033595
100128071_at 2.895654714
100128098_at 2.055105331
100128139_at 1.874028308

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908179_Holly_07.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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