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Sample GSM908181 Query DataSets for GSM908181
Status Public on Mar 04, 2013
Title E176_05_Placebo_4_B
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_05_Placebo_4
treatment: Placebo
weeks of treatment: 4
subject: E176_05
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
100009676_at 2.368815789
10000_at 2.871272078
100049716_at 3.541844422
10004_at 3.359533717
10008_at 2.81241544
100093630_at 9.157425097
10009_at 7.898024485
100101467_at 3.090244778
100113407_at 4.805695974
10011_at 5.907338867
100124700_at 1.334203281
100125288_at 2.668562177
100126784_at 1.981713906
100126791_at 1.986136717
100126793_at 2.829952589
100127891_at 2.647810564
100127983_at 2.815961605
100128071_at 3.918827523
100128098_at 2.0550314
100128139_at 1.784982293

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.




Supplementary file Size Download File type/resource
GSM908181_Holly_09.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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