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Sample GSM908182 Query DataSets for GSM908182
Status Public on Mar 04, 2013
Title E176_07_Placebo_2_B
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_07_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_07
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
100009676_at 2.303360042
10000_at 2.857280865
100049716_at 3.781930302
10004_at 3.590133779
10008_at 2.894434249
100093630_at 8.370682557
10009_at 7.465673189
100101467_at 3.482239268
100113407_at 4.795294023
10011_at 5.193574792
100124700_at 1.778066072
100125288_at 3.340663236
100126784_at 2.069725086
100126791_at 2.118573464
100126793_at 2.912010678
100127891_at 2.702229837
100127983_at 2.44434529
100128071_at 3.836702338
100128098_at 1.937148634
100128139_at 1.693832682

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.




Supplementary file Size Download File type/resource
GSM908182_Holly_10.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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