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Sample GSM908186 Query DataSets for GSM908186
Status Public on Mar 04, 2013
Title E176_07_Placebo_4_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_07_Placebo_4
treatment: Placebo
weeks of treatment: 4
subject: E176_07
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.381281202
10000_at 2.355567473
100049716_at 3.957339354
10004_at 4.104985681
10008_at 4.21103985
100093630_at 8.31973147
10009_at 7.936660944
100101467_at 3.390821256
100113407_at 6.037623489
10011_at 5.473592833
100124700_at 1.747226747
100125288_at 3.106196505
100126784_at 1.828321746
100126791_at 1.71313973
100126793_at 2.304494309
100127891_at 2.742675431
100127983_at 2.542667598
100128071_at 2.650088671
100128098_at 1.923018745
100128139_at 1.602749477

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908186_Holly_14.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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