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Sample GSM908193 Query DataSets for GSM908193
Status Public on Mar 04, 2013
Title E176_14_Leptin_1_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_14_Leptin_1
treatment: Leptin
weeks of treatment: 1
subject: E176_14
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.505833294
10000_at 1.961901062
100049716_at 4.36923836
10004_at 3.626255598
10008_at 4.106144624
100093630_at 7.925487711
10009_at 7.309890149
100101467_at 3.138035809
100113407_at 6.911964029
10011_at 6.260078184
100124700_at 1.714377568
100125288_at 3.17021684
100126784_at 1.929279356
100126791_at 1.883833504
100126793_at 2.381772816
100127891_at 2.620461078
100127983_at 2.611313066
100128071_at 3.067391168
100128098_at 2.381430218
100128139_at 1.715073064

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908193_Holly_22.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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