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Sample GSM908198 Query DataSets for GSM908198
Status Public on Mar 04, 2013
Title E176_12_Placebo_4_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_12_Placebo_4
treatment: Placebo
weeks of treatment: 4
subject: E176_12
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 3.133403652
10000_at 2.096862167
100049716_at 3.350314036
10004_at 3.62102825
10008_at 3.214067367
100093630_at 8.832598554
10009_at 7.6905586
100101467_at 2.961572695
100113407_at 5.625843979
10011_at 7.78063539
100124700_at 1.804637802
100125288_at 3.688452353
100126784_at 2.142524472
100126791_at 2.858947761
100126793_at 2.571031864
100127891_at 2.4710915
100127983_at 2.557568899
100128071_at 3.763631733
100128098_at 2.23876808
100128139_at 1.896580763

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908198_Holly_27.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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