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Sample GSM908218 Query DataSets for GSM908218
Status Public on Mar 04, 2013
Title E176_32_Leptin_2_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_32_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_32
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 3.35646154
10000_at 2.878856497
100049716_at 3.98347847
10004_at 3.616198504
10008_at 3.142394112
100093630_at 6.888794897
10009_at 6.819346123
100101467_at 2.811276772
100113407_at 4.377590629
10011_at 4.546119205
100124700_at 2.036441443
100125288_at 3.923732706
100126784_at 2.712273654
100126791_at 2.462847628
100126793_at 4.069144546
100127891_at 2.751067295
100127983_at 2.176227831
100128071_at 4.16364322
100128098_at 1.901944287
100128139_at 2.52194623

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908218_Holly_50.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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