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Sample GSM908219 Query DataSets for GSM908219
Status Public on Mar 04, 2013
Title E176_29_Placebo_4_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_29_Placebo_4
treatment: Placebo
weeks of treatment: 4
subject: E176_29
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.475189657
10000_at 2.223043828
100049716_at 3.886235446
10004_at 3.500963455
10008_at 4.822054914
100093630_at 8.09967348
10009_at 7.296986588
100101467_at 2.952441653
100113407_at 6.027917877
10011_at 6.22692422
100124700_at 2.270922606
100125288_at 4.08996648
100126784_at 2.004847797
100126791_at 1.691668132
100126793_at 2.978534077
100127891_at 2.620461078
100127983_at 2.563915853
100128071_at 3.729374442
100128098_at 2.146282387
100128139_at 1.734666398

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908219_Holly_51.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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