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Sample GSM908223 Query DataSets for GSM908223
Status Public on Mar 04, 2013
Title E176_31_Leptin_4_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_31_Leptin_4
treatment: Leptin
weeks of treatment: 4
subject: E176_31
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.592112321
10000_at 2.4373635
100049716_at 3.911004716
10004_at 4.425832201
10008_at 4.005379964
100093630_at 7.299642318
10009_at 7.774074714
100101467_at 3.425657505
100113407_at 6.545257552
10011_at 5.733810062
100124700_at 1.560684187
100125288_at 3.717908185
100126784_at 1.847956459
100126791_at 1.890534622
100126793_at 2.628231935
100127891_at 2.599776837
100127983_at 3.174406434
100128071_at 3.165837566
100128098_at 2.191413324
100128139_at 1.700221504

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908223_Holly_55.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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