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Sample GSM908226 Query DataSets for GSM908226
Status Public on Mar 04, 2013
Title E176_32_Leptin_3_B
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_32_Leptin_3
treatment: Leptin
weeks of treatment: 3
subject: E176_32
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
100009676_at 2.231230322
10000_at 2.383579303
100049716_at 3.966803789
10004_at 3.490579075
10008_at 4.491654662
100093630_at 7.657316389
10009_at 7.915861124
100101467_at 3.279059231
100113407_at 6.702589685
10011_at 5.61574289
100124700_at 1.654057725
100125288_at 3.233932763
100126784_at 1.887322458
100126791_at 1.803637373
100126793_at 2.721729531
100127891_at 2.890111234
100127983_at 2.615934003
100128071_at 3.155095374
100128098_at 2.047467719
100128139_at 1.769208451

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.

Supplementary file Size Download File type/resource
GSM908226_Holly_58.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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