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Sample GSM908228 Query DataSets for GSM908228
Status Public on Mar 04, 2013
Title E176_31_Leptin_2_B
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_31_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_31
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15396
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
100009676_at 2.180923196
10000_at 2.361528086
100049716_at 4.09501084
10004_at 4.412488359
10008_at 4.074887528
100093630_at 8.229745953
10009_at 7.783173801
100101467_at 3.408877118
100113407_at 6.641884282
10011_at 5.805263592
100124700_at 1.435065744
100125288_at 3.302848153
100126784_at 1.717265561
100126791_at 1.633949877
100126793_at 2.510813212
100127891_at 2.745087956
100127983_at 2.627251191
100128071_at 3.120814545
100128098_at 2.071202765
100128139_at 1.479521033

Total number of rows: 7906

Table truncated, full table size 166 Kbytes.




Supplementary file Size Download File type/resource
GSM908228_Holly_61.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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