|
| Status |
Public on Apr 03, 2012 |
| Title |
pft1 16C repB |
| Sample type |
RNA |
| |
|
| Source name |
10-day-old pft1-1 seedlings, grown on MS salts agar plates, at 16ºC under continuous light cool-white fluorescent light, 80 micromoles/sec m2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
age: 10-day-old
tissue: seedling
genetic background: Columbia
genotype: pft1-1
growth conditions: 16C
|
| Treatment protocol |
Seedlings were harvested, weighed and frozen in liquid nitrogen before RNA extraction
|
| Growth protocol |
10-day-old seedlings, grown on MS salts agar plates, under continuous light cool-white fluorescent light, 80 micromoles/sec m2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using a plant RNAeasy kit (Qiagen, http://www.qiagen.com) according to manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
The cRNA that was hybridized to the Affymetrix expression arrays (ATH1-121501) as described by the manufacturer (Affymetrix, http://www.affymetrix.com)
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
The expression set was obtained after RMA normalization using the affy package implemented in the R system v2.10
|
| |
|
| Submission date |
Apr 03, 2012 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Sabrina Iñigo |
| E-mail(s) |
sinigo@leloir.org.ar
|
| Phone |
+54(11) 5238-7500
|
| Fax |
+54(11) 5238-7501
|
| URL |
http://www.leloir.org.ar
|
| Organization name |
Fundación Instituto Leloir
|
| Lab |
Plant Molecular Biology
|
| Street address |
Av. Patricias Argentinas 435
|
| City |
Ciudad de Buenos Aires |
| State/province |
Buenos Aires |
| ZIP/Postal code |
C1405BWE |
| Country |
Argentina |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE37014 |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
|
| Relations |
| Reanalyzed by |
GSE118579 |
