|
| Status |
Public on Apr 17, 2012 |
| Title |
CDX treatment, population 2, male 2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila male with paternally transmitted X-chromosome from CDX population 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: male
genotype: derived from LHm population
age: day 14 (4 days adult)
treatment: CDX
population: 2
tissue: whole body
|
| Treatment protocol |
Male-limited X-chromosome evolution is made possible in D. melanogaster by the use of DX females, which have two X-chromosomes that are linked at the centromere. This ensures father-son transmission of the X, but does not affect the transmission of the autosomes. MLX males experienced many generations of father-son transmission of the X-chromosome, potentially enabling adaptation to this altered transmission scheme. CDX males were produced by crossing a control male to a DX female, resulting in one generation of father-son transmission.
|
| Growth protocol |
All populations were maintained using the same culturing protocol as for the ancestral LHm population. Eggs are laid on day 0, adult flies eclose on day 9 or 10, and on day 12, adult flies are mixed between vials, and 16 pairs per vial are randomly selected to produce the next generation. These pairs interact for 48 hours in vials supplemented with live yeast, and then are transferred to new (yeastless) vials, and females are given an 18 hour window in which to lay eggs. At the end of this period, all adult flies are discarded (new day 0). The 48 hour period with controlled density of 16 pairs ensures that all adult flies experience the same conditions while competing for matings (males) or yeast (females).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by Qiagen purification.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
CImpM2-2
|
| Data processing |
The data were analyzed with RMA (Robust Multichip Average) as implemented by the affy package in R 2.9 (BioConductor 2.4) using default settings.
|
| |
|
| Submission date |
Apr 16, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jessica K. Abbott |
| E-mail(s) |
jessica.abbott@biol.lu.se
|
| Organization name |
Lund University
|
| Department |
Dept. of Biology
|
| Lab |
Section for Evolutionary Ecology
|
| Street address |
Sölvegatan 37
|
| City |
Lund |
| ZIP/Postal code |
22362 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE37325 |
Expression profiles of Drosophila melanogaster males with DX mothers and X-chromosomes that were subjected to male-limited evolution |
|
| Relations |
| Reanalyzed by |
GSE119084 |
