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Sample GSM92860 Query DataSets for GSM92860
Status Public on Jun 27, 2006
Title 10 pg GA different samples hybridization 1
Sample type RNA
 
Channel 1
Source name 3T3 fibroblast cell line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification starting from 10 pg of total RNA
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
 
Channel 2
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification starting with 10 pg of total RNA
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 pg of total RNA from each cell line was GA amplified. Ovary DNA was indirectly labeled with Cy5 and 3T3 DNA was indirectly labeled with Cy3 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
 
Submission date Jan 18, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
E-mail(s) subkhankul@hotmail.com
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
ID_REF
VALUE 3T3 vs OV

Data table
ID_REF VALUE
CG_Mm_3000205_1 null
CG_Mm_3000208_1 null
CG_Mm_3000211_1 0.469831442271764
CG_Mm_3000214_1 0.154280113731344
CG_Mm_3000229_1 -0.644803474953505
CG_Mm_3000232_1 -4.63146068872923
CG_Mm_3000235_1 0.0923399011915418
CG_Mm_3000238_1 -2.54249456811083
CG_Mm_3000253_1 1.95678632933039
CG_Mm_3000256_1 null
CG_Mm_3000259_1 null
CG_Mm_3000262_1 -1.14039157666054
CG_Mm_3000277_1 -2.05522742796955
CG_Mm_3000280_1 null
CG_Mm_3000283_1 null
CG_Mm_3000286_1 null
CG_Mm_3000589_1 1.05233773161219
CG_Mm_3000592_1 -0.280027591766987
CG_Mm_3000595_1 2.743196810968
CG_Mm_3000598_1 null

Total number of rows: 21997

Table truncated, full table size 589 Kbytes.




Supplementary data files not provided

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