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Sample GSM92870 Query DataSets for GSM92870
Status Public on Jun 27, 2006
Title 10 pg SMART replicate hybridization 1
Sample type RNA
 
Channel 1
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
 
Channel 2
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica2
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 pg of total RNA was SMART amplified in two parallel reactions. Replica1 was indirectly labeled with Cy3 and replica2 was indirectly labeled with Cy5 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
 
Submission date Jan 18, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
E-mail(s) subkhankul@hotmail.com
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
ID_REF
VALUE Ov vs OV

Data table
ID_REF VALUE
CG_Mm_3000205_1 null
CG_Mm_3000208_1 null
CG_Mm_3000211_1 0.293703851176478
CG_Mm_3000214_1 -0.00306281876722747
CG_Mm_3000229_1 null
CG_Mm_3000232_1 null
CG_Mm_3000235_1 -0.141126839297107
CG_Mm_3000238_1 0.122086819777823
CG_Mm_3000253_1 0.655396461007915
CG_Mm_3000256_1 null
CG_Mm_3000259_1 null
CG_Mm_3000262_1 null
CG_Mm_3000277_1 0.250202980853118
CG_Mm_3000280_1 null
CG_Mm_3000283_1 null
CG_Mm_3000286_1 null
CG_Mm_3000589_1 -0.263189182492771
CG_Mm_3000592_1 -0.232616744540858
CG_Mm_3000595_1 null
CG_Mm_3000598_1 0.0458832630948041

Total number of rows: 21997

Table truncated, full table size 655 Kbytes.




Supplementary data files not provided

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