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Sample GSM92894 Query DataSets for GSM92894
Status Public on Jun 27, 2006
Title 1 ng SMART replicate hybridization 2
Sample type RNA
 
Channel 1
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica2
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
 
Channel 2
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 100 pg of total RNA was SMART amplified in two parallel reactions. Replica1 was indirectly labeled with Cy5 and replica2 was indirectly labeled with Cy3 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
 
Submission date Jan 18, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
E-mail(s) subkhankul@hotmail.com
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
ID_REF
VALUE OV vs OV

Data table
ID_REF VALUE
CG_Mm_3000205_1 0.0561393031226613
CG_Mm_3000208_1 -0.0638004865017003
CG_Mm_3000211_1 -0.157448555637312
CG_Mm_3000214_1 0.0274294979368286
CG_Mm_3000229_1 -0.0554052597533624
CG_Mm_3000232_1 -0.0666514419474263
CG_Mm_3000235_1 -0.072992850326496
CG_Mm_3000238_1 0.173606843442335
CG_Mm_3000253_1 0.100442159673392
CG_Mm_3000256_1 -0.17488227315926
CG_Mm_3000259_1 null
CG_Mm_3000262_1 -0.00314912999734676
CG_Mm_3000277_1 0.084061683289847
CG_Mm_3000280_1 -0.206799746461191
CG_Mm_3000283_1 0.0282670673166405
CG_Mm_3000286_1 -0.0484758431601133
CG_Mm_3000589_1 -0.0331725064021732
CG_Mm_3000592_1 0.191356549718025
CG_Mm_3000595_1 -0.0517234594832839
CG_Mm_3000598_1 0.108321865298385

Total number of rows: 21997

Table truncated, full table size 788 Kbytes.




Supplementary data files not provided

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