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Sample GSM93978 Query DataSets for GSM93978
Status Public on Jun 27, 2006
Title 100 pg GA replicate hybridization 1
Sample type RNA
 
Channel 1
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
 
Channel 2
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification, replica 2
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 100 pg of total RNA was GA amplified in two parallel reactions. Replica1 was indirectly labeled with Cy3 and replica2 was indirectly labeled with Cy5 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
 
Submission date Jan 27, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
E-mail(s) subkhankul@hotmail.com
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
ID_REF
VALUE Ov vs OV

Data table
ID_REF VALUE
CG_Mm_3000205_1 null
CG_Mm_3000208_1 0.0424647124266508
CG_Mm_3000211_1 -0.0340112465835389
CG_Mm_3000214_1 0.116106837480687
CG_Mm_3000229_1 0.231850837028258
CG_Mm_3000232_1 -0.226425157691261
CG_Mm_3000235_1 0.297094724743279
CG_Mm_3000238_1 0.141107361412957
CG_Mm_3000253_1 0.20439823167001
CG_Mm_3000256_1 -0.561928466824368
CG_Mm_3000259_1 null
CG_Mm_3000262_1 0.0320929820567535
CG_Mm_3000277_1 0.089919590604818
CG_Mm_3000280_1 0.0553936093726044
CG_Mm_3000283_1 -0.150763448359248
CG_Mm_3000286_1 null
CG_Mm_3000589_1 -0.0106898853121657
CG_Mm_3000592_1 0.151738992202919
CG_Mm_3000595_1 0.306152621990376
CG_Mm_3000598_1 null

Total number of rows: 21997

Table truncated, full table size 684 Kbytes.




Supplementary data files not provided

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