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Sample GSM93981 Query DataSets for GSM93981
Status Public on Jun 27, 2006
Title 10 pg GA replicate hybridization 2
Sample type RNA
 
Channel 1
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification, replica2
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
 
Channel 2
Source name Ovary_epithelium_cell_line
Organism Mus musculus
Characteristics Product of GA PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 pg of total RNA was GA amplified in two parallel reactions. Replica1 was indirectly labeled with Cy5 and replica2 was indirectly labeled with Cy3 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
 
Submission date Jan 27, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
E-mail(s) subkhankul@hotmail.com
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
ID_REF
VALUE Ov vs Ov

Data table
ID_REF VALUE
CG_Mm_3000205_1 0.025436916319039
CG_Mm_3000208_1 0.821379601485802
CG_Mm_3000211_1 0.5833866029489
CG_Mm_3000214_1 0.285792489999896
CG_Mm_3000229_1 -0.126822197678152
CG_Mm_3000232_1 0.043786985617437
CG_Mm_3000235_1 -0.0904762726185536
CG_Mm_3000238_1 0.410443420271437
CG_Mm_3000253_1 0.754914459838944
CG_Mm_3000256_1 0.648762253307344
CG_Mm_3000259_1 null
CG_Mm_3000262_1 0.334106171340153
CG_Mm_3000277_1 0.12487459556467
CG_Mm_3000280_1 -0.558103573407232
CG_Mm_3000283_1 null
CG_Mm_3000286_1 null
CG_Mm_3000589_1 0.0774321766731459
CG_Mm_3000592_1 -0.0769465946651566
CG_Mm_3000595_1 -0.138680981352253
CG_Mm_3000598_1 -0.738686798473817

Total number of rows: 21997

Table truncated, full table size 746 Kbytes.




Supplementary data files not provided

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