|
| Status |
Public on Jun 14, 2012 |
| Title |
(normal) Ovarian Surface Epithelia_Patient ID 448 |
| Sample type |
RNA |
| |
|
| Source name |
Cell brushings from the ovarian surface epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: LCM-derived
patient id: 448
tissue: Ovarian Surface Epithelium
age: 63
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected from frozen sections using LCM. RNA was isolated with PicoPure RNA isolation KIT (LifeTechnologies). Concentration and Quality of RNA was measured with RNA PicoChip on Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with Ovation Pico WTA System (NuGen) and labeled with Encor Biotin Module (NuGen)
|
| |
|
| Hybridization protocol |
Chips were hybridized to platform GPL570 platform (Affymetrix Human Genome U133 Plus2.0 Array) using Affymetrix HWS KIT and FS450_0004 Protocol
|
| Scan protocol |
Scan on 7G Affimetrix GeneChip scanner
|
| Description |
Gene expression data of cell brushings from the Ovarian Surface Epithelium
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis.
|
| |
|
| Submission date |
Jun 12, 2012 |
| Last update date |
Jun 14, 2012 |
| Contact name |
Loukia Lili |
| E-mail(s) |
loukialili@gmail.com
|
| Phone |
4046422896
|
| Organization name |
The Institute for Next Generation Healthcare
|
| Department |
Icahn School of Medicine at Mount Sinai
|
| Street address |
770 Lexington Ave, 14-07 14th floor
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE38666 |
Molecular Profiling provides evidence of the existence of two functionally distinct classes of ovarian cancer stroma |
|
