|
| Status |
Public on Jan 31, 2013 |
| Title |
amiR-tor20_3d_E |
| Sample type |
RNA |
| |
|
| Source name |
2 week-old transgenic plants; 3 days estradiol-induced plants, amiR-tor 20
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedlings
treatment: 3 days estradiol-treated
cultivar: Arabidopsis Col-0 background, inducible artificial micro RNA
genotype/variation: amiR-tor 20
|
| Treatment protocol |
Two weeks-old transgenic seedlings were transferred to MS plate with 20µM estradiol to induce the overexpression of amiR-tor (consequently, repression of AtTOR levels) under the control of an estradiol-(EST) inducible promoter. Identically treated wild-type Col-0 and pER8 empty-vector (EV) transformed seedlings were served as controls. All the genotypes were also transferred to MS plate without EST as an additional control. Seedlings were harvested after 0, 3 and 6 (amiR-tor lines) days.
|
| Growth protocol |
Transgenic amiR-tor seeds were surfaced sterilized and germinated in MS plates with hygromycin at continuous light.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction performed using Rneasy Plant MiniKit (Qiagene)
|
| Label |
biotin
|
| Label protocol |
Labling procedure performed using MessageAmpTM II-Biotin Enhanced Kit
|
| |
|
| Hybridization protocol |
performed by Atlas Biolabs
|
| Scan protocol |
performed by Atlas Biolabs
|
| Description |
2 week-old transgenic plants (Arabidopsis Col-0 background, inducible artificial micro RNA) estradiol-induced plants for 3 days
Gene expression data from empty inducible vector
|
| Data processing |
The data were analyzed with GCRMA (Wu et al., 2004).
|
| |
|
| Submission date |
Jun 22, 2012 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Camila Caldana |
| E-mail(s) |
camila.caldana@bioetanol.org.br
|
| Phone |
00551935123585
|
| Organization name |
Brazilian Bioethanol Science and Technology Laboratory
|
| Street address |
Rua Giuseppe Máximo Scolfaro, 10.000
|
| City |
Campinas |
| State/province |
Sao Paulo |
| ZIP/Postal code |
13083-970 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE38878 |
Identification of ORB1 target genes using inducible overexpression system |
|
| Relations |
| Reanalyzed by |
GSE118579 |
