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Sample GSM957703 Query DataSets for GSM957703
Status Public on Jul 10, 2012
Title miApoB
Sample type SRA
Source name miApoB
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: liver
age: 8 wk post injection
genotype: wild type
Extracted molecule total RNA
Extraction protocol Liver were removed, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description processed data file = Expression.txt
Data processing Illumina Casava1.8.2 software used for basecalling.
The raw sequencing data produced was processed removing the sequence reads which were of too low quality (only "passing filter" reads were selected). Each read file (sample), in the FASTQ format, was individually aligned against the mouse reference genome (15 May 2012 NCBI build 38.1) using CLC Bio-Genomic Workbench and the expression abundance for each gene (RPKM) was calculated according to Montazavi et al.
Genome_build: mouse reference genome (15 May 2012 NCBI build 38.1)
Supplementary_files_format_and_content: Spreadsheet including annotation of reads to the reference genome
Submission date Jul 09, 2012
Last update date May 15, 2019
Contact name Piotr Maczuga
Organization name UniQure
Street address Meibergdreef 61
City Amsterdam
State/province Nord Holland
ZIP/Postal code 1105BA
Country Netherlands
Platform ID GPL13112
Series (1)
GSE39187 Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy
SRA SRX158349
BioSample SAMN01086229

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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