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Sample GSM966554 Query DataSets for GSM966554
Status Public on Jul 14, 2012
Title MCF_7_vector Rep_2
Sample type RNA
 
Source name MCF-7 with retroviral vector stably transduced
Organism Homo sapiens
Characteristics cell line: MCF-7
cell type: breast cancer cells
transduction: vector control
Treatment protocol pMSCV-miR-374a or empty vector was co-transfected with the pIK packaging plasmid in 293FT cells by using the standard calcium phosphate transfection method. Thirty-six hours after the co-transfection, supernatants were collected and incubated with cells to be infected for 24 hours in the presence of polybrene (2.5 μg/mL). Following infection, puromycin (1.5μg/mL) was used to select stably transduced cells for 10 days.
Growth protocol The MCF-7 breast cancer cells were cultured in complete DMEM medium and supplied with 10% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNeasy RNA isolation kit (Qiagen) following the supplier's protocol.
Label Cy3
Label protocol Labeling was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
 
Hybridization protocol Hybridization was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
Scan protocol Scanning was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
Description MCF-7 with retroviral vector stably transduced, replicate 2; 1.5μg/mL puromycin selection for 10 days.
Data processing Data normalization and transformation were performed using GeneSpring GX 11 (Agilent) by the Shanghai Biochip Corporation.
 
Submission date Jul 13, 2012
Last update date Jul 30, 2013
Contact name Mengfeng Li
E-mail(s) limf@mail.sysu.edu.cn
Organization name Sun Yat-sen University
Department Zhongshan school of medicine
Lab Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
Street address 74# zhongshan 2 Rd.
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platform ID GPL6480
Series (2)
GSE39356 MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer (mRNA dataset)
GSE39359 MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer

Data table header descriptions
ID_REF
VALUE log2 transformed and median shifted signal

Data table
ID_REF VALUE
GE_BrightCorner 16.21514
DarkCorner 2.773148
A_24_P66027 11.95924
A_32_P77178 6.728712
A_23_P212522 10.442824
A_24_P934473 5.8112407
A_24_P9671 14.533171
A_32_P29551 7.7656937
A_24_P801451 8.46954
A_32_P30710 17.077387
A_32_P89523 7.1154838
A_24_P704878 3.641252
A_32_P86028 16.804613
A_24_P470079 5.970277
A_23_P65830 14.035171
A_23_P109143 12.822636
A_24_P595567 4.7942777
A_24_P391591 9.731924
A_24_P799245 2.7581723
A_24_P932757 2.7604344

Total number of rows: 41078

Table truncated, full table size 893 Kbytes.




Supplementary file Size Download File type/resource
GSM966554_MCF_7_vector_Rep_2.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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