|
Status |
Public on Jul 14, 2012 |
Title |
MCF_7_vector Rep_2 |
Sample type |
RNA |
|
|
Source name |
MCF-7 with retroviral vector stably transduced
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 cell type: breast cancer cells transduction: vector control
|
Treatment protocol |
pMSCV-miR-374a or empty vector was co-transfected with the pIK packaging plasmid in 293FT cells by using the standard calcium phosphate transfection method. Thirty-six hours after the co-transfection, supernatants were collected and incubated with cells to be infected for 24 hours in the presence of polybrene (2.5 μg/mL). Following infection, puromycin (1.5μg/mL) was used to select stably transduced cells for 10 days.
|
Growth protocol |
The MCF-7 breast cancer cells were cultured in complete DMEM medium and supplied with 10% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNeasy RNA isolation kit (Qiagen) following the supplier's protocol.
|
Label |
Cy3
|
Label protocol |
Labeling was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
|
|
|
Hybridization protocol |
Hybridization was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
|
Scan protocol |
Scanning was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
|
Description |
MCF-7 with retroviral vector stably transduced, replicate 2; 1.5μg/mL puromycin selection for 10 days.
|
Data processing |
Data normalization and transformation were performed using GeneSpring GX 11 (Agilent) by the Shanghai Biochip Corporation.
|
|
|
Submission date |
Jul 13, 2012 |
Last update date |
Jul 30, 2013 |
Contact name |
Mengfeng Li |
E-mail(s) |
limf@mail.sysu.edu.cn
|
Organization name |
Sun Yat-sen University
|
Department |
Zhongshan school of medicine
|
Lab |
Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
|
Street address |
74# zhongshan 2 Rd.
|
City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510080 |
Country |
China |
|
|
Platform ID |
GPL6480 |
Series (2) |
GSE39356 |
MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer (mRNA dataset) |
GSE39359 |
MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer |
|