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Status |
Public on Jul 14, 2012 |
Title |
MDA-MB-435-Parental_1_Rep 1 |
Sample type |
RNA |
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Source name |
parental MDA-MB-435 from No.1 mouse primary explants
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Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-435 cell type: parental cells cultured from primary explant
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Treatment protocol |
MDA-MB-435 cells were injected into the mammary fat pads of BALB/C nude mice. After 30 days, the animals were sacrificed, pulmonary metastatic nodules were isolated and minced, and cell culture was established using the obtained explants.
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Growth protocol |
The MDA-MB-435 breast cancer cells were cultured in complete DMEM medium and supplied with 10% FBS.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using mirVanaTM miRNA isolation kit (Ambion, Austin, TX) following supplier’s protocol.
|
Label |
Cy3
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Label protocol |
Labeling was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
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Hybridization protocol |
Hybridization was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
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Scan protocol |
Scanning was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
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Description |
MDA-MB-435 primary cells cultured from parental tumor explants within No.1 mouse replicate 1, after 30 days inoculation.
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Data processing |
Data normalization and transformation were performed using GeneSpring GX 11 (Agilent) by the Shanghai Biochip Corporation.
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Submission date |
Jul 13, 2012 |
Last update date |
Jul 30, 2013 |
Contact name |
Mengfeng Li |
E-mail(s) |
limf@mail.sysu.edu.cn
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Organization name |
Sun Yat-sen University
|
Department |
Zhongshan school of medicine
|
Lab |
Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
|
Street address |
74# zhongshan 2 Rd.
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510080 |
Country |
China |
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Platform ID |
GPL11487 |
Series (2) |
GSE39358 |
MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer (miRNA dataset) |
GSE39359 |
MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer |
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