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Sample GSM966564 Query DataSets for GSM966564
Status Public on Jul 14, 2012
Title MDA-MB-435-Parental_2_Rep 2
Sample type RNA
 
Source name parental MDA-MB-435 from No.2 mouse primary explants
Organism Homo sapiens
Characteristics cell line: MDA-MB-435
cell type: parental cells cultured from primary explant
Treatment protocol MDA-MB-435 cells were injected into the mammary fat pads of BALB/C nude mice. After 30 days, the animals were sacrificed, pulmonary metastatic nodules were isolated and minced, and cell culture was established using the obtained explants.
Growth protocol The MDA-MB-435 breast cancer cells were cultured in complete DMEM medium and supplied with 10% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using mirVanaTM miRNA isolation kit (Ambion, Austin, TX) following supplier’s protocol.
Label Cy3
Label protocol Labeling was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
 
Hybridization protocol Hybridization was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
Scan protocol Scanning was performed by the Shanghai Biochip Corporation (http://www.shbiochip.com/) following the standard Agilent protocol.
Description MDA-MB-435 primary cells cultured from parental tumor explants within No.2 mouse replicate 2, after 30 days inoculation.
Data processing Data normalization and transformation were performed using GeneSpring GX 11 (Agilent) by the Shanghai Biochip Corporation.
 
Submission date Jul 13, 2012
Last update date Jul 30, 2013
Contact name Mengfeng Li
E-mail(s) limf@mail.sysu.edu.cn
Organization name Sun Yat-sen University
Department Zhongshan school of medicine
Lab Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
Street address 74# zhongshan 2 Rd.
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platform ID GPL11487
Series (2)
GSE39358 MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer (miRNA dataset)
GSE39359 MiR-374a Promotes Epithelial-Mesenchymal Transition (EMT) and Metastasis of Breast Cancer

Data table header descriptions
ID_REF
VALUE log2 transformed and median shifted signal

Data table
ID_REF VALUE
DarkCorner -3.3180504
NC1_00000197 -3.3180504
NC1_00000215 -3.3180504
NC2_00079215 -3.3180504
NC2_00092197 -3.3180504
NC2_00106057 -3.3180504
NC2_00122731 -3.3180504
NegativeControl -3.3180504
SCorner3 12.395674
bkv-miR-B1-3p -3.3180504
bkv-miR-B1-5p -3.3180504
dmr_285 11.251426
dmr_3 14.207325
dmr_308 -3.3180504
dmr_316 -3.3180504
dmr_31a 9.797282
dmr_6 14.111718
ebv-miR-BART1-3p -3.3180504
ebv-miR-BART1-5p -3.3180504
ebv-miR-BART10 -3.3180504

Total number of rows: 961

Table truncated, full table size 22 Kbytes.




Supplementary file Size Download File type/resource
GSM966564_MDA-MB-435-Parental_2_Rep_2.txt.gz 918.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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