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Sample GSM971338 Query DataSets for GSM971338
Status Public on Mar 25, 2013
Title Bcell_H3K27me1
Sample type SRA
Source name Blood
Organism Homo sapiens
Characteristics tissue: Blood
developmental stage: Adult
genotype/variation: wild type
cell type: Bcell
chip antibody details: Upstate, 07-448
Growth protocol N/A
Extracted molecule genomic DNA
Extraction protocol Purified from whole blood using human Naive B cell isolation kit II kits (Miltenvi #130-091-150)
As described in Barski et al., 2007 (A. Barski, S. Cuddapah, K. Cui, T.Y. Roh, D.E. Schones, Z. Wang, G. Wei, I. Chepelev and K. Zhao, High-resolution profiling of histone methylations in the human genome, Cell 129 (2007), pp. 823-837).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing Illumina Casava1.7 software used for basecalling.
Reads mapped to hg18 using bowtie 0.12.7, with -m 1 allowing one location per read
Mapped reads filtered to allow one read per position
Pol II and p300 binding sites calculated using MACS 1.4.1, -s hg, --p-value=1e-8
H2AZ analyzed with SICER using -w 200 -g 400 -t 0.68 -e 100 -f 150 -p 1e-5
H3K4 analyzed with SICER using -w 200 -g 200 -t 0.68 -e 100 -f 150 -p 1e-5
Brg1 analyzed with SICER using -w 200 -g 600 -t 0.68 -e 100 -f 150 -p 1e-5
H3K27 analyzed with SICER using predicted -w -I 150 -t 0.68 -e 100 -g 0 -f 150 -p 1e-5,
Outermost windows of H3K27 islands were trimmed to maximize the difference between island and non-island bins of 50bp
Genome_build: hg18
Supplementary_files_format_and_content: BED: chromosome, start, end, ReadID, number of mapping locations of read, strand
Supplementary_files_format_and_content: Island: chromosome, start, end, reads in island in control, reads in island in test, pvalue, normalized fold change, FDR
Supplementary_files_format_and_content: bsites (Peak): chromosome, start, end, peak ID, -10(log10(pvalue))
Submission date Jul 20, 2012
Last update date May 15, 2019
Contact name Brian J Abraham
Organization name St. Jude Children's Research Hospital
Department Computational Biology
Street address 262 Danny Thomas Blvd
City Memphis
State/province TN
ZIP/Postal code 38112
Country USA
Platform ID GPL9115
Series (2)
GSE39229 Mapping Epigenomic Type-Specific Differences Occurring During Hematopoiesis
GSE39538 Mapping Epigenomic Type-Specific Differences Occurring During Hematopoiesis [ChIP-Seq]
SRA SRX170351
BioSample SAMN01091976

Supplementary file Size Download File type/resource
GSM971338_Naive_B_cells-H3K27me1.bed.gz 91.7 Mb (ftp)(http) BED
GSM971338_Naive_B_cells-H3K27me1.islands.txt.gz 528.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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