|
| Status |
Public on Aug 07, 2012 |
| Title |
Affymetrix HG-U133 arrays of NIH Epigenomics Roadmap Cell Types; I3 (eye three) stem cells; RNAPrp 24_513980 |
| Sample type |
RNA |
| |
|
| Source name |
I3 (eye three) stem cells; RNAPrp 24_513980
|
| Organism |
Homo sapiens |
| Characteristics |
lineage: embrionic stem cell
medium: TESR
disease: none
passage: 45
line: hES I3 TESR
differentiation_method: NA
batch: hES I3 TESR-1
biomaterial_type: Cell Line
differentiation_stage: undifferentiated
Sex: Female
biomaterial_provider: Harvard University
|
| Treatment protocol |
untreated cultured cells
|
| Growth protocol |
tissue culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA extracted using Qiagen Rneasy kit
|
| Label |
biotin
|
| Label protocol |
As recommended by Affymetrix
|
| |
|
| Hybridization protocol |
As recommended by Affymetrix
|
| Scan protocol |
As recommended by Affymetrix
|
| Description |
sample_term_id: NTR_0000836
assay_term_id: OBI_0001463
nucleic_acid_term_id: SO_0000356
I3 stem cells were cultured using standard methods in TESR media; total RNA was isolated and subject to Affymetrix profiling
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.13328
sample alias: 60
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.3160
|
| Data processing |
Processed data were not provided for this record
|
| |
|
| Submission date |
Aug 06, 2012 |
| Last update date |
Jan 29, 2015 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
| Relations |
| BioSample |
SAMN00119928 |
