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Sample GSM992303 Query DataSets for GSM992303
Status Public on Jan 22, 2015
Title CC with lymph node metastasis, primary tumor PEM-01P
Sample type RNA
 
Source name CC with lymph node metastasis
Organism Homo sapiens
Characteristics tissue: tumor endothelium from cholangiocarcinoma
disease state: CC with lymph node metastasis
Growth protocol Enriched tumor endothelium was obtained by laser capture microdissection
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the PicoPure protocol (Arcturus, Mountain View, CA)
Label biotin
Label protocol RNA was amplified with two linear amplification steps by in vitro transcription using the MEGAscript T7 kit (Ambion, Austin, TX) followed by the labeling step using the BioArray HighYield RNA Transcript Labeling Kit T3 from Enzo Life Sciences (Farmingdale, NY).
 
Hybridization protocol Labeled cRNA was hybridized onto Affymetix GeneChip HG-U133 Plus 2.0 Arrays.
Scan protocol standard Affymetrix protocol
Data processing MAS 5.0
 
Submission date Aug 24, 2012
Last update date Jan 22, 2015
Contact name Xin Wei Wang
E-mail(s) xw3u@nih.gov
Phone 240-760-6858
Organization name National Cancer Institute
Department Laboratory of Human Carcinogenesis
Lab Liver Carcinogenesis Unit
Street address 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-4255
Country USA
 
Platform ID GPL570
Series (1)
GSE40367 Gene expression analysis of liver and colon cancer primary tumors and metastasis

Data table header descriptions
ID_REF
VALUE log2 MAS 5.0 signal

Data table
ID_REF VALUE
1007_s_at 9.608027458
1053_at 5.654554367
117_at 5.480385303
121_at 7.955398083
1255_g_at 3.75061655
1294_at 7.785798073
1316_at 6.593700886
1320_at 3.321928024
1405_i_at 8.816023827
1431_at 3.321928024
1438_at 3.321928024
1487_at 6.952893734
1494_f_at 4.596071243
1552256_a_at 8.337627411
1552257_a_at 7.946781635
1552258_at 5.448411465
1552261_at 4.631844997
1552263_at 5.873749733
1552264_a_at 5.294792652
1552266_at 3.50245142

Total number of rows: 54675

Table truncated, full table size 1213 Kbytes.




Supplementary file Size Download File type/resource
GSM992303_Guoling_Pem_01AC_081607.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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