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Sample GSM992310 Query DataSets for GSM992310
Status Public on Jan 22, 2015
Title CC with lymph node metastasis, metastasis tumor PEM-06M
Sample type RNA
 
Source name CC with lymph node metastasis
Organism Homo sapiens
Characteristics tissue: tumor endothelium from lymph node metastasis
disease state: CC with lymph node metastasis
Growth protocol Enriched tumor endothelium was obtained by laser capture microdissection
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the PicoPure protocol (Arcturus, Mountain View, CA)
Label biotin
Label protocol RNA was amplified with two linear amplification steps by in vitro transcription using the MEGAscript T7 kit (Ambion, Austin, TX) followed by the labeling step using the BioArray HighYield RNA Transcript Labeling Kit T3 from Enzo Life Sciences (Farmingdale, NY).
 
Hybridization protocol Labeled cRNA was hybridized onto Affymetix GeneChip HG-U133 Plus 2.0 Arrays.
Scan protocol standard Affymetrix protocol
Data processing MAS 5.0
 
Submission date Aug 24, 2012
Last update date Jan 22, 2015
Contact name Xin Wei Wang
E-mail(s) xw3u@nih.gov
Phone 240-760-6858
Organization name National Cancer Institute
Department Laboratory of Human Carcinogenesis
Lab Liver Carcinogenesis Unit
Street address 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-4255
Country USA
 
Platform ID GPL570
Series (1)
GSE40367 Gene expression analysis of liver and colon cancer primary tumors and metastasis

Data table header descriptions
ID_REF
VALUE log2 MAS 5.0 signal

Data table
ID_REF VALUE
1007_s_at 9.491822243
1053_at 6.481302738
117_at 7.58744669
121_at 6.971660614
1255_g_at 3.321928024
1294_at 7.784879684
1316_at 5.402192593
1320_at 4.170407772
1405_i_at 8.27453804
1431_at 3.520759583
1438_at 3.321928024
1487_at 6.912640572
1494_f_at 4.464627743
1552256_a_at 9.77674675
1552257_a_at 7.888169765
1552258_at 6.006699562
1552261_at 3.321928024
1552263_at 5.998203754
1552264_a_at 5.033023834
1552266_at 3.321928024

Total number of rows: 54675

Table truncated, full table size 1214 Kbytes.




Supplementary file Size Download File type/resource
GSM992310_Guoling_Pem_06BC_081607.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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