|
| Status |
Public on Jan 22, 2015 |
| Title |
HCC with lung metastasis, primary tumor PEM-13P |
| Sample type |
RNA |
| |
|
| Source name |
HCC with lung metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tumor endothelium from HCC tumor tissue
disease state: HCC with lung metastasis
|
| Growth protocol |
Enriched tumor endothelium was obtained by laser capture microdissection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the PicoPure protocol (Arcturus, Mountain View, CA)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with two linear amplification steps by in vitro transcription using the MEGAscript T7 kit (Ambion, Austin, TX) followed by the labeling step using the BioArray HighYield RNA Transcript Labeling Kit T3 from Enzo Life Sciences (Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized onto Affymetix GeneChip HG-U133 Plus 2.0 Arrays.
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Aug 24, 2012 |
| Last update date |
Jan 22, 2015 |
| Contact name |
Xin Wei Wang |
| E-mail(s) |
xw3u@nih.gov
|
| Phone |
240-760-6858
|
| Organization name |
National Cancer Institute
|
| Department |
Laboratory of Human Carcinogenesis
|
| Lab |
Liver Carcinogenesis Unit
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4255 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE40367 |
Gene expression analysis of liver and colon cancer primary tumors and metastasis |
|
