|
| Status |
Public on Aug 01, 2013 |
| Title |
WT-2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type trichome
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: wild-type
tissue: trichome
|
| Treatment protocol |
N/A
|
| Growth protocol |
Plants were germinated and grown on plates containing Murashige and Skoog salts, pH 5.7, 1% sucrose (w/v), and 0.5% phytogel (w/v) in continuous light at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trichomes were removed with forceps from the fifth and sixth rosette leaves of three-week-old wild-type and gtl1-1 mutant plants according to a previously established protocol (Jakoby et al, 2008). Total RNA was extracted from three biological replicates by a Qiagen plant RNeasy kit, and treated with DNaseI (Takara Bio, Japan) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA probes were labeled using an Affymetrix 3' IVT Express Kit (Affymetrix, Japan)
|
| |
|
| Hybridization protocol |
Hybridized onto Arabidopsis ATH1 Genome GeneChips® (Affymetrix Inc., USA) according to manifucture's instruction (Affymetrix).
|
| Scan protocol |
GeneChips are scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA) and CEL files are generated from DAT files using GeneChip® Operating Software (GCOS) software (Affymetrix Inc., USA).
|
| Description |
Wild type trichome, biological replicate 2
|
| Data processing |
Affy-GUI, normalized by GCRMA.
|
| |
|
| Submission date |
Aug 31, 2012 |
| Last update date |
Aug 01, 2013 |
| Contact name |
Kengo Morohashi |
| E-mail(s) |
morohashi.1@osu.edu
|
| Phone |
614-688-4954
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Lab |
Erich Grotewold
|
| Street address |
1060 Carmack Rd
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (2) |
| GSE40518 |
Comparison of gene expression in Arabidopsis trichome between gtl1-1 mutant and wild type |
| GSE40520 |
GTL1 regulates ploidy-dependent cell growth through direct transcriptional repression of APC/CCS52A1 in Arabidopsis |
|
