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Status |
Public on Aug 01, 2013 |
Title |
gtl1-2 |
Sample type |
RNA |
|
|
Source name |
gtl1-1 mutant trichome
|
Organism |
Arabidopsis thaliana |
Characteristics |
genotype/variation: gtl1-1 mutant tissue: trichome
|
Treatment protocol |
N/A
|
Growth protocol |
Plants were germinated and grown on plates containing Murashige and Skoog salts, pH 5.7, 1% sucrose (w/v), and 0.5% phytogel (w/v) in continuous light at 22°C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trichomes were removed with forceps from the fifth and sixth rosette leaves of three-week-old wild-type and gtl1-1 mutant plants according to a previously established protocol (Jakoby et al, 2008). Total RNA was extracted from three biological replicates by a Qiagen plant RNeasy kit, and treated with DNaseI (Takara Bio, Japan) following the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
RNA probes were labeled using an Affymetrix 3' IVT Express Kit (Affymetrix, Japan)
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|
|
Hybridization protocol |
Hybridized onto Arabidopsis ATH1 Genome GeneChips® (Affymetrix Inc., USA) according to manifucture's instruction (Affymetrix).
|
Scan protocol |
GeneChips are scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA) and CEL files are generated from DAT files using GeneChip® Operating Software (GCOS) software (Affymetrix Inc., USA).
|
Description |
gtl1-1 mutant trichome, biological replicate 2
|
Data processing |
Affy-GUI, normalized by GCRMA.
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|
|
Submission date |
Aug 31, 2012 |
Last update date |
Aug 01, 2013 |
Contact name |
Kengo Morohashi |
E-mail(s) |
morohashi.1@osu.edu
|
Phone |
614-688-4954
|
Organization name |
The Ohio State University
|
Department |
Molecular Genetics
|
Lab |
Erich Grotewold
|
Street address |
1060 Carmack Rd
|
City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43210 |
Country |
USA |
|
|
Platform ID |
GPL198 |
Series (2) |
GSE40518 |
Comparison of gene expression in Arabidopsis trichome between gtl1-1 mutant and wild type |
GSE40520 |
GTL1 regulates ploidy-dependent cell growth through direct transcriptional repression of APC/CCS52A1 in Arabidopsis |
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