|
| Status |
Public on Aug 05, 2013 |
| Title |
ATCC 17978 vs 17978hm Technical repeat |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Untreated cells
|
| Organism |
Acinetobacter baumannii |
| Characteristics |
strain: ATCC 17978
genotype/variation: wild type
|
| Growth protocol |
0.25% agar + Mueller-Hinton medium 8hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using TriZol extraction followed by spin-column purification and on-column DNAseI treatment (all Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
Cy3 and Cy5 labelling using Agilent Fairplay Microarray Labelling kit (Stratagene)
|
| |
|
| Channel 2 |
| Source name |
Motile cells
|
| Organism |
Acinetobacter baumannii |
| Characteristics |
strain: 17978hm
genotype/variation: knockout derivative of strain ATCC17978
phenotype: hyper-motile on semi-solid MH media
|
| Growth protocol |
0.25% agar + Mueller-Hinton medium 8hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using TriZol extraction followed by spin-column purification and on-column DNAseI treatment (all Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Cy3 and Cy5 labelling using Agilent Fairplay Microarray Labelling kit (Stratagene)
|
| |
|
| |
| Hybridization protocol |
The Ramaciotti Centre, UNSW, Sydney
|
| Scan protocol |
The Ramaciotti Centre, UNSW, Sydney
|
| Description |
Technical repeat sample 3
|
| Data processing |
The data obtained from replicate probes per gene was processed using Agilent Genespring GX version 9. The processed data was subseqeuntly analyzed using the Significance Analysis of Micrroarray data (Tusher et al. 2001) with a false discovery rate of less than 5%.
|
| |
|
| Submission date |
Sep 07, 2012 |
| Last update date |
Aug 05, 2013 |
| Contact name |
Bart Eijkelkamp |
| E-mail(s) |
bart.eijkelkamp@adelaide.edu.au
|
| Organization name |
University of Adelaide
|
| Street address |
North Tce
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL11113 |
| Series (1) |
| GSE40681 |
Comparative transcriptional analysis of Acinetobacter baumannii strain ATCC 17978 and strain 17978hm |
|
