|
| Status |
Public on Aug 29, 2013 |
| Title |
C57Bl6/J_mammary glandF_Lin- |
| Sample type |
RNA |
| |
|
| Source name |
Normal mammary gland
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype/variation: Apc+/+
histology: Normal
sorted population: Lin-
|
| Treatment protocol |
Tumors/mammary glands were collected, single cell suspensions were prepared, immuno labeled and FACS sorted. A total of 10,000 cells were collected for each population.
|
| Growth protocol |
Animals were housed in SPF conditions and fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNeasy Micro kit (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Two-cycle Labelling kit according to Affymetrix’s instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Standard Affymetrix protocols.
|
| Description |
MECs93
|
| Data processing |
After GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization, and median-polish summarization were done using Partek Genomics Suite Software (Partek, Inc.).
|
| |
|
| Submission date |
Sep 07, 2012 |
| Last update date |
Aug 29, 2013 |
| Contact name |
Claudia Gaspar |
| E-mail(s) |
cscgaspar@gmail.com
|
| Organization name |
ErasmusMC
|
| Department |
Experimental Pathology
|
| Street address |
Dr. Molenwaterplein 40, PO Box 2040
|
| City |
Rotterdam |
| ZIP/Postal code |
3000 CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE40702 |
Gene signature of adult mammary stem cells and mammary cancer stem cells (Affymetrix) |
| GSE40715 |
Gene signature of adult mammary stem cells and mammary cancer stem cells |
|
