|
Status |
Public on Aug 29, 2013 |
Title |
C57Bl6/J_mammary glandF_Lin-CD29+CD24+ |
Sample type |
RNA |
|
|
Source name |
Normal mammary gland
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL6/J genotype/variation: Apc+/+ histology: Normal sorted population: Lin-CD29+CD24+
|
Treatment protocol |
Tumors/mammary glands were collected, single cell suspensions were prepared, immuno labeled and FACS sorted. A total of 10,000 cells were collected for each population.
|
Growth protocol |
Animals were housed in SPF conditions and fed ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with RNeasy Micro kit (QIAGEN).
|
Label |
Biotin
|
Label protocol |
Two-cycle Labelling kit according to Affymetrix’s instructions.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Standard Affymetrix protocols.
|
Description |
MECs82
|
Data processing |
After GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization, and median-polish summarization were done using Partek Genomics Suite Software (Partek, Inc.).
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|
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Submission date |
Sep 07, 2012 |
Last update date |
Aug 29, 2013 |
Contact name |
Claudia Gaspar |
E-mail(s) |
cscgaspar@gmail.com
|
Organization name |
ErasmusMC
|
Department |
Experimental Pathology
|
Street address |
Dr. Molenwaterplein 40, PO Box 2040
|
City |
Rotterdam |
ZIP/Postal code |
3000 CA |
Country |
Netherlands |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE40702 |
Gene signature of adult mammary stem cells and mammary cancer stem cells (Affymetrix) |
GSE40715 |
Gene signature of adult mammary stem cells and mammary cancer stem cells |
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