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Sample GSM999484 Query DataSets for GSM999484
Status Public on Aug 29, 2013
Title C57Bl6/J_mammary glandF_Lin-CD29+CD24+
Sample type RNA
 
Source name Normal mammary gland
Organism Mus musculus
Characteristics strain: C57BL6/J
genotype/variation: Apc+/+
histology: Normal
sorted population: Lin-CD29+CD24+
Treatment protocol Tumors/mammary glands were collected, single cell suspensions were prepared, immuno labeled and FACS sorted. A total of 10,000 cells were collected for each population.
Growth protocol Animals were housed in SPF conditions and fed ad libitum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with RNeasy Micro kit (QIAGEN).
Label Biotin
Label protocol Two-cycle Labelling kit according to Affymetrix’s instructions.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Standard Affymetrix protocols.
Description MECs82
Data processing After GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization, and median-polish summarization were done using Partek Genomics Suite Software (Partek, Inc.).
 
Submission date Sep 07, 2012
Last update date Aug 29, 2013
Contact name Claudia Gaspar
E-mail(s) cscgaspar@gmail.com
Organization name ErasmusMC
Department Experimental Pathology
Street address Dr. Molenwaterplein 40, PO Box 2040
City Rotterdam
ZIP/Postal code 3000 CA
Country Netherlands
 
Platform ID GPL1261
Series (2)
GSE40702 Gene signature of adult mammary stem cells and mammary cancer stem cells (Affymetrix)
GSE40715 Gene signature of adult mammary stem cells and mammary cancer stem cells

Data table header descriptions
ID_REF
VALUE GCRMA signal.

Data table
ID_REF VALUE
1415670_at 10.6699
1415671_at 11.3627
1415672_at 11.8971
1415673_at 7.3277
1415674_a_at 10.2375
1415675_at 9.0253
1415676_a_at 12.1138
1415677_at 8.4361
1415678_at 10.9191
1415679_at 12.2691
1415680_at 8.7833
1415681_at 9.4175
1415682_at 8.0051
1415683_at 12.0331
1415684_at 8.9859
1415685_at 7.6205
1415686_at 9.6398
1415687_a_at 12.0196
1415688_at 11.2133
1415689_s_at 8.8673

Total number of rows: 45101

Table truncated, full table size 815 Kbytes.




Supplementary file Size Download File type/resource
GSM999484_MECs82.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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