|
| Status |
Public on Aug 29, 2013 |
| Title |
C57Bl6/J_mammary glandF_Lin-CD29hiCD24+ |
| Sample type |
RNA |
| |
|
| Source name |
Normal mammary gland
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6/J
genotype/variation: Apc+/+
histology: Normal
population: Lin-CD29hiCD24+
|
| Treatment protocol |
Tumors/mammary glands were collected, single cell suspensions were prepared, immuno labeled and FACS sorted. A total of 10,000 cells were collected for each population.
|
| Growth protocol |
Animals were housed in SPF conditions and fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNeasy Micro kit (QIAGEN).
|
| Label |
n/a
|
| Label protocol |
cDNAs were generated using High Capacity RNA-to-cDNA kit (Applied Biosystems) and employed for a pre-amplification step in Taqman PreAmp Master Mix for 14 cycles (Applied Biosysytems), according to manufacturer’s protocol. Real-Time PCR was performed by the 7900HT Fast Real-Time PCR System (Applied Biosystems). Replicate CT values were averaged.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions.
For the normalization two technical replicates of ACTB were used.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
|
| |
|
| Submission date |
Sep 07, 2012 |
| Last update date |
Aug 29, 2013 |
| Contact name |
Claudia Gaspar |
| E-mail(s) |
cscgaspar@gmail.com
|
| Organization name |
ErasmusMC
|
| Department |
Experimental Pathology
|
| Street address |
Dr. Molenwaterplein 40, PO Box 2040
|
| City |
Rotterdam |
| ZIP/Postal code |
3000 CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL2850 |
| Series (2) |
| GSE40704 |
Gene signature of adult mammary stem cells and mammary cancer stem cells (RT_PCR) |
| GSE40715 |
Gene signature of adult mammary stem cells and mammary cancer stem cells |
|
