Asah2 N-acylsphingosine amidohydrolase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Asah2provided by RGD
Official Full Name: N-acylsphingosine amidohydrolase 2provided by RGD
Primary source: RGD:69410
See related: EnsemblRapid:ENSRNOG00000012196 AllianceGenome:RGD:69410
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables dihydroceramidase activity and phytoceramidase activity. Involved in response to organic substance and sphingolipid metabolic process. Located in extracellular space; mitochondrion; and plasma membrane. Is integral component of plasma membrane. Colocalizes with membrane raft. Orthologous to several human genes including ASAH2B (N-acylsphingosine amidohydrolase 2B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 32.8), Adrenal (RPKM 25.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q52

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (239278994..239386598, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (229865662..229973253, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (250557042..250665083, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Low-grade elevation of palmitate and lipopolysaccharide synergistically induced beta-cell damage via inhibition of neutral ceramidase.
Title: Low-grade elevation of palmitate and lipopolysaccharide synergistically induced β-cell damage via inhibition of neutral ceramidase.
Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
Title: Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
mucin box and signal/anchor sequence of a rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membranes of mammalian cells
Title: The mucin box and signal/anchor sequence of rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membrane.
insights into the actions of Pseudomonas aeruginosa CDases and eukaryotic neutral CDases as an exotoxin and mediators of sphingolipid signaling are also revealed, respectively
Title: Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
Chronic activation of neutral ceramidase protects beta-cells against cytokine-induced apoptosis.
Title: Chronic activation of neutral ceramidase protects beta-cells against cytokine-induced apoptosis.
Data show that conserved hydrophobic amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization, and enzyme activity of neutral ceramidase.
Title: Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization and enzyme activity of neutral ceramidase.
The presence of bile salts is important for maintaining high intraluminal levels of Alk-SMase and N-CDase, two key enzymes for hydrolysis of sphingomyelin in the gut.
Title: Effects of bile diversion in rats on intestinal sphingomyelinases and ceramidase.
Intestinal ceramidase thus is a neutral ceramidase 2 released by bile salts and resistant to pancreatic proteases and well suited to metabolize ceramide formed from sphingolipids to generate other bioactive sphingolipid messengers.
Title: Rat intestinal ceramidase: purification, properties, and physiological relevance.
the present study identified a novel amidase sequence containing a critical serine residue that may function as a nucleophile in the hydrolytic attack on the amide bond present in ceramide
Title: Identification of a novel amidase motif in neutral ceramidase.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables N-acylsphingosine amidohydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables N-acylsphingosine amidohydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-acylsphingosine amidohydrolase activity	IEA Inferred from Electronic Annotation more info
enables N-acylsphingosine amidohydrolase activity	ISO Inferred from Sequence Orthology more info
enables N-acylsphingosine amidohydrolase activity	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables ceramidase activity	IEA Inferred from Electronic Annotation more info
enables dihydroceramidase activity	IDA Inferred from Direct Assay more info	PubMed
enables phytoceramidase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cytokine stimulus	ISO Inferred from Sequence Orthology more info
involved_in ceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in ceramide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ceramide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ceramide catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ceramide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in ceramide metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid digestion	ISO Inferred from Sequence Orthology more info
involved_in lipid digestion	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-chain fatty acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in sphingosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphingosine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sphingosine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in sphingosine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in sphingosine metabolic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in caveola	ISO Inferred from Sequence Orthology more info
located_in caveola	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in extracellular exosome	ISS Inferred from Sequence or Structural Similarity more info
is_active_in extracellular region	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
NOT located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neutral ceramidase

Names: N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; NCDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase

NP_446098.1

EC 3.5.1.23

XP_006231325.1

EC 3.5.1.23

XP_006231332.1

EC 3.5.1.23

XP_008758542.1

EC 3.5.1.23

XP_017444136.1

EC 3.5.1.23

XP_017444137.1

EC 3.5.1.23

XP_017444139.1

EC 3.5.1.23

XP_038962801.1

EC 3.5.1.23

XP_038962806.1

EC 3.5.1.23

XP_038962807.1

EC 3.5.1.23

XP_038962839.1

EC 3.5.1.23

XP_038962916.1

EC 3.5.1.23

XP_038962949.1

EC 3.5.1.23

XP_038962978.1

EC 3.5.1.23

XP_038963012.1

EC 3.5.1.23

XP_038963044.1

EC 3.5.1.23

XP_038963081.1

EC 3.5.1.23

XP_063118252.1

EC 3.5.1.23

XP_063118305.1

EC 3.5.1.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053646.2 → NP_446098.1 neutral ceramidase

See identical proteins and their annotated locations for NP_446098.1

Status: VALIDATED

Source sequence(s)

AB057433, JAXUCZ010000001

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Related

ENSRNOP00000073116.2, ENSRNOT00000077135.3

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

239278994..239386598 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039107021.2 → XP_038962949.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039106988.2 → XP_038962916.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_006231263.3 → XP_006231325.1 neutral ceramidase isoform X1

See identical proteins and their annotated locations for XP_006231325.1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_006231270.3 → XP_006231332.1 neutral ceramidase isoform X1

See identical proteins and their annotated locations for XP_006231332.1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_063262182.1 → XP_063118252.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102
XM_008760320.4 → XP_008758542.1 neutral ceramidase isoform X1

See identical proteins and their annotated locations for XP_008758542.1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039107153.2 → XP_038963081.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039106879.2 → XP_038962807.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039107084.2 → XP_038963012.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_063262235.1 → XP_063118305.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102
XM_039107050.2 → XP_038962978.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Related

ENSRNOP00000104976.1, ENSRNOT00000128918.1

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039107116.2 → XP_038963044.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_017588650.3 → XP_017444139.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039106911.2 → XP_038962839.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039106878.2 → XP_038962806.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_017588648.3 → XP_017444137.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_039106873.2 → XP_038962801.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal
XM_017588647.3 → XP_017444136.1 neutral ceramidase isoform X1

UniProtKB/Swiss-Prot

Q91XT9

UniProtKB/TrEMBL

A0A8L2Q853, A6I102

Conserved Domains (2) summary

pfam04734
Location:83 → 589

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:591 → 758

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal