Mpst mercaptopyruvate sulfurtransferase [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Mpstprovided by RGD
Full Name: mercaptopyruvate sulfurtransferaseprovided by RGD
Primary source: RGD:620065
See related: Ensembl:ENSRNOG00000000185 AllianceGenome:RGD:620065
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Mst
Summary: Enables identical protein binding activity and thiosulfate sulfurtransferase activity. Involved in animal organ development and transsulfuration. Located in cytosol and mitochondrion. Biomarker of type 2 diabetes mellitus. Orthologous to human MPST (mercaptopyruvate sulfurtransferase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 572.5), Liver (RPKM 385.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q34

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (111836079..111843651)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (109955581..109963155)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (119626636..119631317)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects cerebral endothelial cells against oxygen-glucose deprivation/reoxygenation-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.
Title: 3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects cerebral endothelial cells against oxygen-glucose deprivation/reoxygenation-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.
The study revealed a significant role of MPST in hydrogen sulfide metabolism in the pancreas in experimental model of acute pancreatitis.
Title: Hydrogen sulfide formation in experimental model of acute pancreatitis.
Presented is alternative pathway of production of hydrogen sulfide and/or polysulfides from (poly)sulfurated catalytic-site cysteine of reaction intermediates of MST via thioredoxin reduction.
Title: Alternative pathway of H(2)S and polysulfides production from sulfurated catalytic-cysteine of reaction intermediates of 3-mercaptopyruvate sulfurtransferase.
observations indicate that 3MST is probably not responsible for the increased production of H2S following permanent middle cerebral artery occlusion.
Title: Brain 3-Mercaptopyruvate Sulfurtransferase (3MST): Cellular Localization and Downregulation after Acute Stroke.
3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function.
Title: MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.
Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, cysteine aminotransferase (CAT) and mercaptopyruvate sulfurtransferase (MPST).
Title: Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.
3MST exists in the neurons of medullary respiratory nuclei and its expression can be up-regulated by chronic intermittent hypoxia in adult rat
Title: Chronic intermittent hypoxia promotes expression of 3-mercaptopyruvate sulfurtransferase in adult rat medulla oblongata.
SiRNA silencing of 3-MPST reduced basal bioenergetic parameters and prevented the stimulating effect of 3-MP on mitochondrial bioenergetics.
Title: Intramitochondrial hydrogen sulfide production by 3-mercaptopyruvate sulfurtransferase maintains mitochondrial electron flow and supports cellular bioenergetics.
Thus, sulfur oxides are suggested to release in the redox cycle of persulfide of mercaptopyruvate sulfurtransferase.
Title: Is novel signal transducer sulfur oxide involved in the redox cycle of persulfide at the catalytic site cysteine in a stable reaction intermediate of mercaptopyruvate sulfurtransferase?
3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) are localized to vascular endothelium in the thoracic aorta and produce hydrogen sulfide.
Title: Vascular endothelium expresses 3-mercaptopyruvate sulfurtransferase and produces hydrogen sulfide.

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General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-mercaptopyruvate sulfurtransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3-mercaptopyruvate sulfurtransferase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables thiosulfate sulfurtransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables thiosulfate sulfurtransferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in hydrogen sulfide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in hydrogen sulfide biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in kidney development	IDA Inferred from Direct Assay more info	PubMed
involved_in liver development	IDA Inferred from Direct Assay more info	PubMed
involved_in spinal cord development	IEP Inferred from Expression Pattern more info	PubMed
involved_in transsulfuration	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 3-mercaptopyruvate sulfurtransferase

NP_620198.1

EC 2.8.1.2

XP_038934288.1

EC 2.8.1.2

XP_063119029.1

EC 2.8.1.2

XP_063119030.1

EC 2.8.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_138843.2 → NP_620198.1 3-mercaptopyruvate sulfurtransferase

See identical proteins and their annotated locations for NP_620198.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

P97532

UniProtKB/TrEMBL

A6HSJ8

Related

ENSRNOP00000000201.2, ENSRNOT00000000201.6

Conserved Domains (1) summary

COG2897
Location:1 → 279

SseA; 3-mercaptopyruvate sulfurtransferase SseA, contains two rhodanese domains [Inorganic ion transport and metabolism]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

111836079..111843651

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039078360.2 → XP_038934288.1 3-mercaptopyruvate sulfurtransferase isoform X2

UniProtKB/TrEMBL

A6HSJ9

Conserved Domains (1) summary

cd01448
Location:10 → 139

TST_Repeat_1; Thiosulfate sulfurtransferase (TST), N-terminal, inactive domain. TST contains 2 copies of the Rhodanese Homology Domain; this is the 1st repeat, which does not contain the catalytically active Cys residue. The role of the 1st repeat is uncertain, but it ...
XM_063262959.1 → XP_063119029.1 3-mercaptopyruvate sulfurtransferase isoform X1

UniProtKB/Swiss-Prot

P97532

UniProtKB/TrEMBL

A6HSJ8
XM_063262960.1 → XP_063119030.1 3-mercaptopyruvate sulfurtransferase isoform X1

UniProtKB/Swiss-Prot

P97532

UniProtKB/TrEMBL

A6HSJ8