Pcbd1 pterin-4 alpha-carbinolamine dehydratase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pcbd1provided by RGD
Official Full Name: pterin-4 alpha-carbinolamine dehydratase 1provided by RGD
Primary source: RGD:3263
See related: EnsemblRapid:ENSRNOG00000000566 AllianceGenome:RGD:3263
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pcd; Phs; DCoH; Pcbd; TCF1
Summary: This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. [provided by RefSeq, Jan 2014]
Expression: Biased expression in Liver (RPKM 770.0) and Kidney (RPKM 464.0) See more
Orthologs: human mouse all
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Genomic context

Location:: 20q11

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (29573362..29587181)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (29037545..29044322)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (30689690..30696465)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rat Pcbd1 is a moonlighting protein that has dehydratase activity in addition to functioning as a cofactor which regulates HNF1alpha.
Title: Identity of 4a-carbinolamine dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins.
a point mutation at the DCoH1 tetramer interface, Thr 51 Ser, overcomes the dissociation barrier of the homotetramer and increases the interaction with HNF-1alpha.
Title: Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1α .

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General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 4-alpha-hydroxytetrahydrobiopterin dehydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 4-alpha-hydroxytetrahydrobiopterin dehydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables 4-alpha-hydroxytetrahydrobiopterin dehydratase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables phenylalanine 4-monooxygenase activity	ISO Inferred from Sequence Orthology more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in L-phenylalanine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in biological_process	ND No biological Data available more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in tetrahydrobiopterin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tyrosine biosynthetic process, by oxidation of phenylalanine	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: pterin-4-alpha-carbinolamine dehydratase

Names: 4-alpha-hydroxy-tetrahydropterin dehydratase; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); dimerization cofactor of HNF1; phenylalanine hydroxylase-stimulating protein; pterin carbinolamine dehydratase; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha

NP_001007602.1

EC 4.2.1.96

XP_006256476.1

EC 4.2.1.96

XP_063135155.1

EC 4.2.1.96

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001007601.2 → NP_001007602.1 pterin-4-alpha-carbinolamine dehydratase

See identical proteins and their annotated locations for NP_001007602.1

Status: VALIDATED

Source sequence(s)

DV724713, FM060466, FM067129

UniProtKB/Swiss-Prot

P61459

UniProtKB/TrEMBL

A0A8L2PZL1, A6K3Y5

Related

ENSRNOP00000100247.1, ENSRNOT00000130263.1

Conserved Domains (1) summary

cd00914
Location:24 → 99

PCD_DCoH_subfamily_b; PCD_DCoH: The bifunctional protein pterin-4alpha-carbinolamine dehydratase (PCD), also known as DCoH (dimerization cofactor of hepatocyte nuclear factor-1), is both a transcription activator and a metabolic enzyme. DCoH stimulates gene expression by ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086038.1 Reference GRCr8

Range

29573362..29587181

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063279085.1 → XP_063135155.1 pterin-4-alpha-carbinolamine dehydratase isoform X1

UniProtKB/Swiss-Prot

P61459

UniProtKB/TrEMBL

A6K3Y5

Related

ENSRNOP00000086732.1, ENSRNOT00000107746.3
XM_006256414.2 → XP_006256476.1 pterin-4-alpha-carbinolamine dehydratase isoform X1

See identical proteins and their annotated locations for XP_006256476.1

UniProtKB/Swiss-Prot

P61459

UniProtKB/TrEMBL

A0A8L2PZL1, A6K3Y5

Conserved Domains (1) summary

cd00914
Location:24 → 99

PCD_DCoH_subfamily_b; PCD_DCoH: The bifunctional protein pterin-4alpha-carbinolamine dehydratase (PCD), also known as DCoH (dimerization cofactor of hepatocyte nuclear factor-1), is both a transcription activator and a metabolic enzyme. DCoH stimulates gene expression by ...