Rock2 Rho-associated coiled-coil containing protein kinase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rock2provided by RGD
Official Full Name: Rho-associated coiled-coil containing protein kinase 2provided by RGD
Primary source: RGD:3590
See related: EnsemblRapid:ENSRNOG00000004496 AllianceGenome:RGD:3590
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: ROK; ROCK-II
Summary: Predicted to enable Rho-dependent protein serine/threonine kinase activity; endopeptidase activator activity; and protease binding activity. Involved in several processes, including positive regulation of cardiac muscle hypertrophy; positive regulation of connective tissue replacement; and positive regulation of cytokine production. Acts upstream of or within Rho protein signal transduction. Predicted to be located in cytoplasmic ribonucleoprotein granule; cytosol; and spindle pole centrosome. Predicted to be active in several cellular components, including Schaffer collateral - CA1 synapse; glutamatergic synapse; and postsynaptic density. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 488.3), Lung (RPKM 320.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q16

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (45407823..45502773)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (39679116..39774033)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (42180864..42289910)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CircHECTD1 promoted MIRI-associated inflammation via inhibiting miR-138-5p and upregulating ROCK2.
Title: CircHECTD1 promoted MIRI-associated inflammation via inhibiting miR-138-5p and upregulating ROCK2.
Increased ROCK1 not ROCK2 in circulating leukocytes in rats with myocardial ischemia/reperfusion.
Title: Increased ROCK1 not ROCK2 in circulating leukocytes in rats with myocardial ischemia/reperfusion.
Ghrelin suppresses migration of macrophages via inhibition of ROCK2 under chronic intermittent hypoxia.
Title: Ghrelin suppresses migration of macrophages via inhibition of ROCK2 under chronic intermittent hypoxia.
Expression of miR-133a-5p and ROCK2 in Heart in Methamphetamine-Induced Rats and Intervention of Rhynchophylline.
Title: Expression of miR-133a-5p and ROCK2 in Heart in Methamphetamine-Induced Rats and Intervention of Rhynchophylline.
Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling.
Title: Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling.
3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects cerebral endothelial cells against oxygen-glucose deprivation/reoxygenation-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.
Title: 3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects cerebral endothelial cells against oxygen-glucose deprivation/reoxygenation-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.
these results demonstrate that miR335 promotes stress granule formation and inhibits apoptosis by reducing ROCK2 expression in acute ischemic stroke.
Title: miR‑335 promotes stress granule formation to inhibit apoptosis by targeting ROCK2 in acute ischemic stroke.
Suggest an essential role for Rock1/2 activity in the insulin signaling and glucose homeostasis improvement in exercising skeletal muscle.
Title: Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle.
Together, the results of this study reveal a novel mechanism through which RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton
Title: RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton.
Inhibition of ROCK2 promotes autophagy and reduces the injury in the hippocampus, leading to the recovery of learning and memory ability following subarachnoid hemorrhage
Title: ROCK2 regulates autophagy in the hippocampus of rats after subarachnoid hemorrhage.

Submit: New GeneRIF Correction See all GeneRIFs (59)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BI279627 (e-PCR)
- UniSTS:245008

AW531154 (e-PCR)
- UniSTS:245082

AW823633 (e-PCR)
- UniSTS:208783

D6Rat34 (e-PCR)
- UniSTS:118972

RH143143 (e-PCR)
- UniSTS:232390

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables endopeptidase activator activity	IEA Inferred from Electronic Annotation more info
enables endopeptidase activator activity	ISO Inferred from Sequence Orthology more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	TAS Traceable Author Statement more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protease binding	IEA Inferred from Electronic Annotation more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in aortic valve morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in blood vessel diameter maintenance	ISO Inferred from Sequence Orthology more info
involved_in canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in cellular response to acetylcholine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to testosterone stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to testosterone stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to transforming growth factor beta stimulus	ISO Inferred from Sequence Orthology more info
involved_in centrosome duplication	IEA Inferred from Electronic Annotation more info
acts_upstream_of centrosome duplication	ISO Inferred from Sequence Orthology more info
involved_in centrosome duplication	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome condensation	TAS Traceable Author Statement more info	PubMed
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cortical actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in cortical actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in epithelial to mesenchymal transition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in mRNA destabilization	ISO Inferred from Sequence Orthology more info
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in modulation by host of viral process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of bicellular tight junction assembly	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of bicellular tight junction assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of biomineral tissue development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein localization to lysosome	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein localization to lysosome	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neural tube closure	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of amyloid-beta formation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of amyloid-beta formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of centrosome duplication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of centrosome duplication	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of connective tissue growth factor production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of connective tissue replacement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of connective tissue replacement	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fibroblast growth factor production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to early endosome	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to early endosome	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of stress fiber assembly	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of angiotensin-activated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cellular response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of establishment of endothelial barrier	IEA Inferred from Electronic Annotation more info
involved_in regulation of establishment of endothelial barrier	ISO Inferred from Sequence Orthology more info
involved_in regulation of keratinocyte differentiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of keratinocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of nervous system process	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of synapse maturation	ISO Inferred from Sequence Orthology more info
involved_in response to angiotensin	ISO Inferred from Sequence Orthology more info
involved_in response to ischemia	ISO Inferred from Sequence Orthology more info
involved_in response to transforming growth factor beta	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to transforming growth factor beta	ISO Inferred from Sequence Orthology more info
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in smooth muscle contraction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic ribonucleoprotein granule	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic active zone	ISO Inferred from Sequence Orthology more info
located_in spindle pole centrosome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: rho-associated protein kinase 2

Names: ROKalpha; Rho-associated coiled-coil forming kinase 2; RhoA - binding serine/threosine kinase alpha (ROK - alpha); p150 ROK-alpha; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; rhoA-binding kinase 2

NP_037154.2

EC 2.7.11.1

XP_006239971.1

EC 2.7.11.1

XP_038967738.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013022.2 → NP_037154.2 rho-associated protein kinase 2

Status: VALIDATED

Source sequence(s)

BF398321, CB691694, CK475383, JAXUCZ010000006, U38481

UniProtKB/Swiss-Prot

Q62868

UniProtKB/TrEMBL

A0A0G2K5N6

Related

ENSRNOP00000073496.1, ENSRNOT00000090816.3

Conserved Domains (7) summary

cd11638
Location:505 → 571

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:92 → 354

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1261 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1151 → 1257

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

pfam08912
Location:979 → 1046

Rho_Binding; Rho Binding

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

45407823..45502773

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006239909.5 → XP_006239971.1 rho-associated protein kinase 2 isoform X2

UniProtKB/TrEMBL

F1LQT3

Related

ENSRNOP00000088194.2, ENSRNOT00000119147.2

Conserved Domains (7) summary

cd11638
Location:505 → 571

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:92 → 354

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1261 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1151 → 1257

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

pfam08912
Location:979 → 1046

Rho_Binding; Rho Binding
XM_039111810.2 → XP_038967738.2 rho-associated protein kinase 2 isoform X1