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    Nit1 nitrilase 1 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 289222, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Nit1provided by RGD
    Official Full Name
    nitrilase 1provided by RGD
    Primary source
    RGD:727821
    See related
    EnsemblRapid:ENSRNOG00000003881 AllianceGenome:RGD:727821
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    The protein encoded by this gene is a member of the nitrilase enzyme superfamily. Nitrilases are nonpeptidic C-N hydrolases. In mammals, nitrilases form small molecule C-N bonds such as acid amides, carbamates and ureas. Nit1-deficient mice display phenotypes including increased cell proliferation, resistance to DNA damage, and an increased number of N-nitrosomethylbenzylamine-induced murine forestomach tumors, suggesting a tumor suppressor function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
    Expression
    Biased expression in Kidney (RPKM 940.4), Adrenal (RPKM 606.4) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Nit1 in Genome Data Viewer
    Location:
    13q24
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (86302334..86305770, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (83769888..83773324, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (89722370..89725806, complement)

    Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene ubiquitin-fold modifier conjugating enzyme 1 Neighboring gene G protein subunit gamma 5, pseudogene 1 Neighboring gene death effector domain-containing Neighboring gene prefoldin subunit 2 Neighboring gene kelch domain containing 9 Neighboring gene nectin cell adhesion molecule 4

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes. Barglow KT, et al. Biochemistry, 2008 Dec 23. PMID 19053248, Free PMC Article
    2. Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. Semba S, et al. J Biol Chem, 2006 Sep 22. PMID 16864578
    3. Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione. Peracchi A, et al. Proc Natl Acad Sci U S A, 2017 Apr 18. PMID 28373563, Free PMC Article
    4. Proteomic characterization of the human sperm nucleus. de Mateo S, et al. Proteomics, 2011 Jul. PMID 21630459
    5. The nitrilase superfamily: classification, structure and function. Pace HC, et al. Genome Biol, 2001. PMID 11380987, Free PMC Article

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC95151

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables deaminated glutathione amidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables deaminated glutathione amidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in amide catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in amide catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    deaminated glutathione amidase
    Names
    dGSH amidase
    nitrilase homolog 1
    NP_001076049.1
    NP_001292575.1
    NP_001292576.1
    NP_872609.2
    XP_038946439.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001082580.1 → NP_001076049.1  deaminated glutathione amidase isoform b

      See identical proteins and their annotated locations for NP_001076049.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region, which results in initiation of translation from a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus compared to isoform a. Variants 2 and 4 encode the same isoform.
      Source sequence(s)
      BC087146, FM045849, JAXUCZ010000013
      UniProtKB/TrEMBL
      A0A8L2Q1A7, A6JFY4
      Related
      ENSRNOP00000096370.2, ENSRNOT00000117822.2
      Conserved Domains (1) summary
      cd07572
      Location:13 → 280
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    2. NM_001305646.1 → NP_001292575.1  deaminated glutathione amidase isoform c

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform c, which is shorter than isoform a.
      Source sequence(s)
      BC087146, JAXUCZ010000013
      UniProtKB/TrEMBL
      A0A8L2Q1A7
      Conserved Domains (1) summary
      cd07572
      Location:43 → 310
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    3. NM_001305647.1 → NP_001292576.1  deaminated glutathione amidase isoform b

      See identical proteins and their annotated locations for NP_001292576.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus than isoform a. Variants 2 and 4 encode the same isoform.
      Source sequence(s)
      BC087146, CV073582
      UniProtKB/TrEMBL
      A0A8L2Q1A7, A6JFY4
      Conserved Domains (1) summary
      cd07572
      Location:13 → 280
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    4. NM_182668.2 → NP_872609.2  deaminated glutathione amidase isoform a

      See identical proteins and their annotated locations for NP_872609.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (a).
      Source sequence(s)
      BC087146, JAXUCZ010000013
      UniProtKB/Swiss-Prot
      Q5PQK6, Q7TQ94
      UniProtKB/TrEMBL
      A0A8L2Q1A7
      Related
      ENSRNOP00000005194.6, ENSRNOT00000005194.7
      Conserved Domains (1) summary
      cd07572
      Location:49 → 316
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086031.1 Reference GRCr8

      Range
      86302334..86305770 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039090511.2 → XP_038946439.1  deaminated glutathione amidase isoform X1

      Conserved Domains (1) summary
      cl11424
      Location:13 → 175
      nitrilase; Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q7TQ94.2 GenPept UniProtKB/Swiss-Prot:Q7TQ94

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
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