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    WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]

    Gene ID: 7465, updated on 28-Oct-2024

    Summary

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    Official Symbol
    WEE1provided by HGNC
    Official Full Name
    WEE1 G2 checkpoint kinaseprovided by HGNC
    Primary source
    HGNC:HGNC:12761
    See related
    Ensembl:ENSG00000166483 MIM:193525; AllianceGenome:HGNC:12761
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    WEE1A; WEE1hu
    Summary
    This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in gall bladder (RPKM 23.7), esophagus (RPKM 19.4) and 22 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See WEE1 in Genome Data Viewer
    Location:
    11p15.4
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (9573670..9589985)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (9657890..9674136)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (9595217..9611532)

    Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:9480928-9481563 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4406 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4407 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4408 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3126 Neighboring gene ZNF143 antisense RNA 1 Neighboring gene zinc finger protein 143 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3127 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:9537259-9537799 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:9551203-9551929 Neighboring gene CRISPRi-validated cis-regulatory element chr11.1006 Neighboring gene H3K27ac hESC enhancer GRCh37_chr11:9584901-9585400 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4409 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3128 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:9594443-9595295 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3131 Neighboring gene small nucleolar RNA U13 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3132 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3133 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4410 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4411 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4412 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:9622289-9622842 Neighboring gene H3K27ac hESC enhancer GRCh37_chr11:9631697-9632224 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4415 Neighboring gene ribosomal protein L23a pseudogene 65 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3134 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4416 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4417 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4418 Neighboring gene uncharacterized LOC124902628

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. WEE1 accumulation and deregulation of S-phase proteins mediate MLN4924 potent inhibitory effect on Ewing sarcoma cells. Mackintosh C, et al. Oncogene, 2013 Mar 14. PMID 22641220
    2. Tumour suppressive effects of WEE1 gene silencing in neuroblastomas. Tashnizi AH, et al. J Cancer Res Ther, 2016 Jan-Mar. PMID 27072241
    3. Constitutive Wee1 activity in adult brain neurons with M phase-type alterations in Alzheimer neurodegeneration. Tomashevski A, et al. J Alzheimers Dis, 2001 Apr. PMID 12214061
    4. Wee1 inhibition potentiates Wip1-dependent p53-negative tumor cell death during chemotherapy. Clausse V, et al. Cell Death Dis, 2016 Apr 14. PMID 27077811, Free PMC Article
    5. Human wee1 kinase is directly transactivated by and increased in association with c-Fos/AP-1: rheumatoid synovial cells overexpressing these genes go into aberrant mitosis. Kawasaki H, et al. Oncogene, 2003 Oct 9. PMID 14534529

    See all (240) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identification of DNA methylation-regulated WEE1 with potential implications in prognosis and immunotherapy for low-grade glioma.
      Title: Identification of DNA methylation-regulated WEE1 with potential implications in prognosis and immunotherapy for low-grade glioma.
    2. In silico and structure-based evaluation of deleterious mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
      Title: In silico and structure-based evaluation of deleterious mutations identified in human Chk1, Chk2, and Wee1 protein kinase.
    3. Circulating RNA ZFR promotes hepatocellular carcinoma cell proliferation and epithelial-mesenchymal transition process through miR-624-3p/WEE1 axis.
      Title: Circulating RNA ZFR promotes hepatocellular carcinoma cell proliferation and epithelial-mesenchymal transition process through miR-624-3p/WEE1 axis.
    4. E2F8 exerts cancer-promoting effects by transcriptionally activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition in suppressing lung adenocarcinoma.
      Title: E2F8 exerts cancer-promoting effects by transcriptionally activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition in suppressing lung adenocarcinoma.
    5. Wee1 promotes cell proliferation and imatinib resistance in chronic myeloid leukemia via regulating DNA damage repair dependent on ATM-gammaH2AX-MDC1.
      Title: Wee1 promotes cell proliferation and imatinib resistance in chronic myeloid leukemia via regulating DNA damage repair dependent on ATM-γH2AX-MDC1.
    6. ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models.
      Title: ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models.
    7. Mitotic Checkpoints and the Role of WEE1 Inhibition in Head and Neck Squamous Cell Carcinoma.
      Title: Mitotic Checkpoints and the Role of WEE1 Inhibition in Head and Neck Squamous Cell Carcinoma.
    8. Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR-101-3p/Wee1 axis.
      Title: Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR-101-3p/Wee1 axis.
    9. Inhibiting WEE1 and IKK-RELA Crosstalk Overcomes TNFalpha Resistance in Head and Neck Cancers.
      Title: Inhibiting WEE1 and IKK-RELA Crosstalk Overcomes TNFα Resistance in Head and Neck Cancers.
    10. WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma.
      Title: WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma.
    Submit: New GeneRIF Correction See all GeneRIFs (132)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Vpr vpr Prolonged cell cycle arrest in the G2 phase induced by HIV-1 Vpr results in the depletion of Wee1 kinase PubMed
    vpr HIV-1 Vpr binds to Wee1. Amino acids 291-369 in Wee1 are required for the binding between Vpr and Wee1. Vpr stimulates Wee1 kinase activity PubMed
    vpr HIV-1 Vpr-induced cell cycle arrest results in increased levels of Wee-1 kinase in synchronized HeLa cells; G2 arrest by Vpr is correlated with delayed degradation of Wee-1, and Cdc2 activity is reduced at G2/M in the presence of Vpr PubMed
    vpr HIV-1 Vpr induced cell cycle arrest in the G2 phase is mediated predominately through the Wee1 kinase in fission yeast PubMed
    vpr Overexpression of Wee-1 kinase suppresses HIV-1 Vpr-mediated apoptosis PubMed
    vpr Rupture of HIV-1 Vpr-induced nuclear envelope herniations produces transient loss of the subcellular compartmentalization of Wee1, Cdc25C, cyclin B1, and presumably other soluble cellular components, resulting in their release into the cytoplasm PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ16446, DKFZp686I18166

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables non-membrane spanning protein tyrosine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein tyrosine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein tyrosine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein tyrosine kinase activity TAS
    Traceable Author Statement
    more info
    		  
    Process Evidence Code Pubs
    involved_in G2/M transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in establishment of cell polarity IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in microtubule cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of G1/S transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
    		  
    involved_in negative regulation of G2/M transition of mitotic cell cycle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of G2/M transition of mitotic cell cycle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in neuron projection morphogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of DNA replication TAS
    Traceable Author Statement
    more info
    		  
    involved_in protein phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    wee1-like protein kinase
    Names
    WEE1 homolog
    WEE1+ homolog
    protein kinase
    wee1A kinase
    NP_001137448.1
    NP_003381.1
    XP_047283495.1
    XP_054225826.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001143976.2 → NP_001137448.1  wee1-like protein kinase isoform 2

      See identical proteins and their annotated locations for NP_001137448.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) has a different first exon and 5' UTR, compared to variant 1. This difference causes translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus, compared to isoform 1.
      Source sequence(s)
      AC011979, AK122837, BQ024511
      Consensus CDS
      CCDS44536.1
      UniProtKB/Swiss-Prot
      P30291
      Related
      ENSP00000299613.5, ENST00000299613.10
      Conserved Domains (2) summary
      cd14138
      Location:79 → 354
      PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a
      pfam00069
      Location:85 → 355
      Pkinase; Protein kinase domain

    2. NM_003390.4 → NP_003381.1  wee1-like protein kinase isoform 1

      See identical proteins and their annotated locations for NP_003381.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC011979, AC122179, BC051831, BQ024511, U10564
      Consensus CDS
      CCDS7800.1
      UniProtKB/Swiss-Prot
      B3KVE1, D3DQV0, P30291
      UniProtKB/TrEMBL
      Q6NSL2, Q86V29
      Related
      ENSP00000402084.2, ENST00000450114.7
      Conserved Domains (2) summary
      cd14138
      Location:293 → 568
      PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a
      pfam00069
      Location:299 → 569
      Pkinase; Protein kinase domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

      Range
      9573670..9589985
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047427539.1 → XP_047283495.1  wee1-like protein kinase isoform X1

      Related
      ENSP00000506667.1, ENST00000681684.1

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060935.1 Alternate T2T-CHM13v2.0

      Range
      9657890..9674136
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054369851.1 → XP_054225826.1  wee1-like protein kinase isoform X1

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P30291.2 GenPept UniProtKB/Swiss-Prot:P30291

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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