ARHGAP35 Rho GTPase activating protein 35 [Homo sapiens (human)] - Gene

Summary

Official Symbol: ARHGAP35provided by HGNC
Official Full Name: Rho GTPase activating protein 35provided by HGNC
Primary source: HGNC:HGNC:4591
See related: Ensembl:ENSG00000160007 MIM:605277; AllianceGenome:HGNC:4591
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
Summary: The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Expression: Broad expression in testis (RPKM 28.9), brain (RPKM 18.7) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 19q13.32

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	19	NC_000019.10 (46860997..47005077)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	19	NC_060943.1 (49686835..49830977)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	19	NC_000019.9 (47364254..47508334)

Chromosome 19 - NC_000019.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP, and Dok1.
Title: Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP, and Dok1.
ARHGAP35 is a novel factor disrupted in human developmental eye phenotypes.
Title: ARHGAP35 is a novel factor disrupted in human developmental eye phenotypes.
Tumor-derived ARHGAP35 mutations enhance the Galpha13-Rho signaling axis in human endometrial cancer.
Title: Tumor-derived ARHGAP35 mutations enhance the Gα(13)-Rho signaling axis in human endometrial cancer.
Identification of an inhibitory domain in GTPase-activating protein p190RhoGAP responsible for masking its functional GAP domain.
Title: Identification of an inhibitory domain in GTPase-activating protein p190RhoGAP responsible for masking its functional GAP domain.
The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in GnRH neuronal development: Evidence from patients with idiopathic hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity assay.
Title: The p190 RhoGAPs, ARHGAP35, and ARHGAP5 are implicated in GnRH neuronal development: Evidence from patients with idiopathic hypogonadotropic hypogonadism, zebrafish, and in vitro GAP activity assay.
Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition.
Title: Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition.
p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer.
Title: p190A inactivating mutations cause aberrant RhoA activation and promote malignant transformation via the Hippo-YAP pathway in endometrial cancer.
Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signaling.
Title: Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signaling.
p190A RhoGAP induces CDH1 expression and cooperates with E-cadherin to activate LATS kinases and suppress tumor cell growth.
Title: p190A RhoGAP induces CDH1 expression and cooperates with E-cadherin to activate LATS kinases and suppress tumor cell growth.
Tau regulates the microtubule-dependent migration of glioblastoma cells via the Rho-ROCK signaling pathway.
Title: Tau regulates the microtubule-dependent migration of glioblastoma cells via the Rho-ROCK signaling pathway.

Submit: New GeneRIF Correction See all GeneRIFs (41)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide meta-analysis identifies variants associated with platinating agent susceptibility across populations EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope transmembrane glycoprotein gp41	env	The 3S motif of HIV-1 gp41 activates p190 RhoGAP	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH79947 (e-PCR)
- UniSTS:89347

D18S1154 (e-PCR), detects polymorphism
- UniSTS:9244

D19S851 (e-PCR)
- UniSTS:10802

D19S540 (e-PCR)
- UniSTS:149075

STS-N25336 (e-PCR)
- UniSTS:48472

WI-9140 (e-PCR)
- UniSTS:14425

RH15918 (e-PCR)
- UniSTS:75371

RH77962 (e-PCR)
- UniSTS:36720

RH68444 (e-PCR)
- UniSTS:26780

RH67855 (e-PCR)
- UniSTS:30382

RH12331 (e-PCR)
- UniSTS:58590

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	TAS Traceable Author Statement more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in axon guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in axonal fasciculation	ISS Inferred from Sequence or Structural Similarity more info
involved_in camera-type eye development	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in central nervous system neuron axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in establishment or maintenance of actin cytoskeleton polarity	ISS Inferred from Sequence or Structural Similarity more info
involved_in forebrain development	IEA Inferred from Electronic Annotation more info
involved_in mammary gland development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of Rho protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of vascular permeability	IEA Inferred from Electronic Annotation more info
involved_in neural tube closure	IEA Inferred from Electronic Annotation more info
involved_in neuron projection guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cilium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin polymerization or depolymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell size	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of small GTPase mediated signal transduction	TAS Traceable Author Statement more info
involved_in wound healing, spreading of cells	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in ciliary basal body	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho GTPase-activating protein 35

Names: glucocorticoid receptor DNA-binding factor 1; glucocorticoid receptor repression factor 1; rho GAP p190A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_047014.2 RefSeqGene

Range

5001..149081

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_004491.5 → NP_004482.4 rho GTPase-activating protein 35

See identical proteins and their annotated locations for NP_004482.4

Status: REVIEWED

Source sequence(s)

AB051509, AC008755, AC008895, BE871213, CA429690, CX163309

Consensus CDS

CCDS46127.1

UniProtKB/Swiss-Prot

A7E2A4, Q14452, Q9C0E1, Q9NRY4

UniProtKB/TrEMBL

A0A2X0SFV3

Related

ENSP00000500409.1, ENST00000672722.1

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000019.10 Reference GRCh38.p14 Primary Assembly

Range

46860997..47005077

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_024451473.2 → XP_024307241.1 rho GTPase-activating protein 35 isoform X1

UniProtKB/TrEMBL

A0A2X0SFV3

Conserved Domains (4) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

cl02570
Location:1247 → 1380

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cl27030
Location:158 → 250

Ras; Ras family

RNA

XR_002958305.2 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

NC_060943.1 Alternate T2T-CHM13v2.0

Range

49686835..49830977

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054320752.1 → XP_054176727.1 rho GTPase-activating protein 35 isoform X1

RNA

XR_008485136.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_024342.1: Suppressed sequence

Description

NM_024342.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.